[1]祁海龙,王斯璐.慢性牙周炎伴咬合创伤患者龈沟液炎性因子表达及与骨代谢指标的相关性研究[J].现代检验医学杂志,2021,36(05):164-168.[doi:10.3969/j.issn.1671-7414.2021.05.036]
　QI Hai-long,WANG Si-lu.Expression of Inflammatory Factors in Gingival CrevicularFluid and Its Correlation with Bone Metabolism in Patients withChronic Periodontitis and Occlusal Trauma[J].Journal of Modern Laboratory Medicine,2021,36(05):164-168.[doi:10.3969/j.issn.1671-7414.2021.05.036]
点击复制

慢性牙周炎伴咬合创伤患者龈沟液炎性因子表达及与骨代谢指标的相关性研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年05期
页码:
164-168
栏目:
检验与临床
出版日期:
2021-10-14

文章信息/Info

Title:
Expression of Inflammatory Factors in Gingival CrevicularFluid and Its Correlation with Bone Metabolism in Patients withChronic Periodontitis and Occlusal Trauma
文章编号:
1671-7414（2021）05-164-05
作者:
祁海龙1;  王斯璐2
（1. 宝鸡市中医院, 陕西宝鸡 721000; 2. 渭南市第一医院, 陕西渭南 714000）
Author(s):
QI Hai-long1;  WANG Si-lu2
（1.Baoji City Hospital of Traditional Chinese Medicine ,Shaanxi Baoji 721000 ,China；2. the First Hospital of Weinan，Shaanxi Weinan 714000，China）
关键词:
慢性牙周炎; 咬合创伤; 龈沟液; 炎性因子; 骨代谢
分类号:
R781.42；R446.19
DOI:
10.3969/j.issn.1671-7414.2021.05.036
文献标志码:
A
摘要:
目的　观察慢性牙周炎伴咬合创伤患者龈沟液炎性因子表达情况，并分析与骨代谢指标的相关性。方法　选取2020年1~4月收治的52例慢性牙周炎伴咬合创伤患者为伴咬伤组，另选取同期慢性牙周炎52例为不伴咬伤组。伴咬伤组和不伴咬伤组均于治疗前采用酶联免疫吸附法进行龈沟液中炎性因子(TNF-α，IL-1β，CRP和IL-6)和骨代谢指标(BALP，CTX-1和tPINP)检测，采用放射免疫法进行骨代谢指标BGP表达值检测。比较伴咬伤组和不伴咬伤组龈沟液炎性因子、骨代谢指标表达差异，并采用Pearson相关系数分析描述龈沟液炎性因子表达及与骨代谢指标的相关性。结果　伴咬伤组的龈沟液炎性因子(TNF-α，CRP，IL-1β和IL-6)表达值、骨代谢指标(BGP，CTX1和tPINP)表达值均高于不伴咬伤组，差异均有统计学意义(t = 5.499 ~ 28.161，均P<0.05)。伴咬伤组的骨代谢指标(BGP)表达值高于不伴咬伤组，差异有统计学意义(t = 4.054，P<0.05)。龈沟液炎性因子(TNF-α，CRP，IL-1β和IL-6)表达值与龈沟液BALP表达值呈负相关性(r = - 0.813 ~ -0.694，均P<0.05)，与龈沟液BGP表达值呈正相关(r =0.708 ~ 0.767，均P<0.05)，与龈沟液CTX1表达值呈正相关(r =0.709 ~ 0.791，均P<0.05)，与龈沟液tPINP表达值呈正相关(r = 0.695 ~ 0.842，均P<0.05)。结论　伴咬合创伤可加重慢性牙周炎患者机体炎性反应，而伴咬合创伤导致的炎性反应也可对慢性牙周炎患者骨代谢水平产生影响，伴咬合创伤对慢性牙周炎病情的促进作用需给予临床重视。
Abstract:
Objective　To observe the expression of inflammatory factors in gingival crevicular fluid (GCF) of patients withchronic periodontitis and occlusal trauma and the correlation with bone metabolism. Methods　From January to April 2020, 52patients with chronic periodontitis and occlusal trauma were selected as mixed bite group, and 52 patients with chronicperiodontitis in the same period were selected as non mixed bite group. Before treatment, the inflammatory factors (TNF - α,IL-1β,CRP and IL-6) and bone metabolism indexes (BALP,CTX-1 and tPINP) in gingival crevicular fluid were detected by enzymelinkedimmunosorbent assay (ELISA) and radioimmunoassay (RIA) respectively. The expression of inflammatory factors andbone metabolism indexes in gingival crevicular fluid were compared between the mixed group and the non mixed group. Pearsonrank correlation method was used to analyze the expression of inflammatory factors in gingival crevicular fluid and its correlationwith bone metabolism indexes. Results　The inflammatory factors of gingival crevicular fluid(TNF-α,IL-1β,CRP and IL-6),bone metabolism index(BALP,CTX-1 and tPINP) in the mixed bite group were all higher than those in the mixed bite group ,thedifferences were statistically significant(t = 5.499 ~ 28.161, all P<0.05), and bone metabolism index (BGP) in the mixed bitegroup were higher than those in the mixed bite group ,the differences were statistically significant(t = 4.054, P<0.05). It wasnegatively correlated with BALP expression in gingival crevicular fluid (r = -0.813~ - 0.694，all P<0.05), positively correlatedwith BGP expression in gingival crevicular fluid (r= 0.708 ~ 0.767，all P<0.05), positively correlated with CTX1 expression ingingival crevicular fluid (r = 0.709 ~ 0.791，all P<0.05), and positively correlated with tPINP expression in gingival crevicularfluid (r = 0.695 ~ 0.842，all P<0.05). Conclusion　Combined with occlusal trauma could aggravate the inflammatory responseof chronic periodontitis patients, and the inflammatory response caused by combined occlusal trauma could also affect the bonemetabolism level of patients with chronic periodontitis. Clinical attention should be paid to the promotion effect of combinedocclusal trauma on the condition of chronic periodontitis.

参考文献/References:

[1] 沈妍欣，郭淑娟，吴亚菲．慢性牙周炎的氧化应激及抗氧化治疗研究进展[J]. 中华口腔医学杂志，2016，51（7）：442-446．SHEN Yanxin, GUO Shujuan, WU Yafei，et al.Oxidative stress and antioxidant therapy of chronicperiodontitis [J]. Chinese Journal of Stomatology，2016,51 (7)：442-446.
[2] 刘洪臣，赵彦平，李世东．咬合创伤性牙髓炎临床分析[J]．口腔颌面修复学杂志, 2000,1(1):40-42.LIU Hongchen，ZHAO Yanping，LI Shidong，etal.Traumatic occlussion pulpitis caused [J]．ChineseJournal of Prosthodontics March, 2000,1(1):40-42.
[3] HOWARD P S, KUCICH U, TALIWAL R, et al.Mechanical forces alter extracellular matrix synthesisby human periodontal ligament fibroblasts[J]. Journalof Periodontal Research, 1998, 33(8): 500-508.
[4] 刘晨, 毕良佳. 肿瘤坏死因子-α 在慢性牙周炎中的研究进展[J]. 中华老年口腔医学杂志，2019,17(5)：308-313.LIU Chen, BI Liangjia. Research progress of tumornecrosis factor-α in chronic periodontitis [J]. ChineseJournal of Geriatric Dentistry,2019,17 (5):308-313.
[5] 蔡春芳．重度牙周炎伴前牙缺失应用不同材料牙周夹板治疗后血清氧化应激、骨代谢及炎症反应指标变化[J]. 实用口腔医学杂志,2019,35(4):519-523.CAI Chunfang. The changes of serum oxidativestress, bone metabolism and inflammatory responsein patients with severe periodontitis and anteriorteeth loss after treatment with periodontal splintof different materials [J]. Journal of PracticalStomatology,2019,35(4):519-523.
[6] 李立，张晨．米诺环素辅助治疗对慢性牙周炎患者血清及龈沟液骨代谢及相关炎症因子的影响[J]. 中国合理用药探索,2019,16 (11):115-118.LI Li，ZHANG Chen. Effect of minocycline-assistedtreatment on serum and gingival crevicular fluidbone metabolism and related inflammatory factors inpatients with chronic periodontitis [J]. China LicensedPharmacist,2019,16 (11):115-118.
[7] 陈小香，谭新，邓伟民．老年男性骨密度与身体成分相关性的研究[J]. 中国骨质疏松杂志,2017,23(10):1296-1299．CHEN Xiaoxiang，TAN Xin，DENG Weimin，etal. The relationship between bone mineral density andbody composition in old men [J]. Chinese Journal ofOsteoporosis,2017,23 (10):1296-1299.
[8] LAMBA A K, TANDON S, FARAZ F, et al. Effectof periodontal disease on electromyographic activityof muscles of mastication: A cross-sectional study[J].Journal of Oral Rehabilitation, 2020, 47(5): 599-605.
[9] 刘东妮．咬合调整对牙周炎正畸治疗患者牙周状况的影响[J]. 医疗装备,2020,33(8):120-121.LIU Dongni. Effect of occlusal adjustments on theperiodontal status of patients with periodontitis afterorthodontic treatment [J]. Chinese Journal of MedicalDevice,2020,33 (8):120-121.
[10] 秦勤，秦德川，郑嵘，等．正畸力作用下龈沟液中碱性成纤维细胞生长因子和白介素-6 表达变化的研究[J]. 泰山医学院学报,2018,39 (9):983-985.QIN Qin，QIN Dechuan，ZHENG Rong，et al.The expression of basic fibroblast growth factorand interleukin-6 level during orthodontic force ingingival crevicular fluid[J]. Journal of Taishan MedicalCollege,2018,39 (9):983-985.
[11] 王杨洋, 赵婵媛, 张海龙, 等. 益气升阳固齿汤联合奥硝唑治疗慢性牙周炎疗效以及对骨保护素水平的影响[J]. 中华中医药学刊,2018,36(8):2012-2015.WANG Yangyang，ZHAO Chanyuan，ZHANGHailong，et al. Effect of Yiqi Shengyang Guchidecoction combined with ornidazole in treatment ofchronic periodontitis and the effect on levels of boneprotection [J]. Chinese Archives of Traditional ChineseMedicine，2018，36 (8)：2012-2015.
[12] 王琳璇，李永明，邱亚，等．肿瘤坏死因子-α在正畸牙移动中作用的研究进展[J]．口腔医学，2018，38（7）：654-658．WANG Linxuan, LI Yongming,QIU Ya, et al. Researchprogress of tumor necrosis factor-α in orthodontictooth movement[J]. Stomatology,2018,38(7):654-658.
[13] 姚瑶，刘焕燕，阎利勇．P 物质与IL-1β 于固定正畸牙龈沟液中的表达分析[J]. 系统医学,2018,3(13):141-143.YAO Yao, LIU Huanyan, YAN Liyong, et al. Analysisof expression of substance P and IL-1β in gingivalcrevicular fluid of orthodontic teeth [J]. SystemsMedicine, 2018, 3(13):141-143.
[14] 叶婵娟. 炎症介质介导牙周炎促进早产发生的研究进展[J]. 临床口腔医学杂志,2020,36(4):249-252.YE Chanjuan. Research progress of periodontitismediated by inflammatory mediators to promotepremature birth [J]. Journal of Clinical Dentistry，2020,36(4):249-252.
[15] JUNG-YOON C，CHANG-HYUK C，KI-YEONP, et al. High-mobility group box 1 is responsiblefor monosodium urate crystal-induced inflammationin human U937 macrophages[J]. Biochemical andBiophysical Research Communications, 2018, 503(4):3248-3255.
[16] ZHENG Wenjie, YAO Min, FANG Miao, et al. Abnormalexpression of HMGB-3 is significantly associatedwith malignant transformation of hepatocytes[J]. WorldJournal of Gastroenterology, 2018, 24(32): 3650-3662.
[17] 杨捷, 王耀志, 沈晓丽, 等. 超声刮治联合替硝唑冲洗对慢性牙周炎患者龈沟液中炎性因子、氧化应激及骨代谢相关因子的影响[J]. 宁夏医科大学学报,2018,40 (11):1329-1331.YANG Jie，WANG Yaozhi，SHEN Xiaoli，et al. Effectsof ultrasonic curettage combined with tinidazole washingon inflammatory factors, oxidative stress and bone metabolismrelated factors in gingival crevicular fluid of patientswith chronic periodontitis [J]. Journal of Ningxia MedicalUniversity，2018，40 (11)：1329-1331.
[18] 刘伟, 吕迪, 肖言栋. 龈沟液中脂联素水平与糖尿病牙周炎患者病程进展的相关性[J]. 西部医学,2019,31(5):778-781.LIU Wei，L? Di，XIAO Yandong，et al. Correlationbetween adiponectin levels in gingival crevicular fluidand the course of diabetic periodontitis [J]. MedicalJournal of West China,2019,31(5):778-781.
[19] LUCIA D W, ANTIKA E J, LEE Y H, et al. Dietaryphlorizin enhances osteoblastogenic bone formationthrough enhancing β-catenin activity via GSK-3βinhibition in a model of senile osteoporosis[J]. TheJournal of Nutritional Biochemistry, 2017, 49(32): 484-493.
[20] CHEN Weiwen, POCOCK N. Male osteoporosisawareness in the elderly: an analysis of Dual-EnergyX-Ray absorptiometry use in Australia between 1995and 2015[J]. Journal of Clinical Densitometry, 2018,21(1): 105-109.
[21] 庞晓霞, 廖湘凌, 王敏娜, 等. 慢性牙周炎患者炎症程度、抗氧化、胰岛素抵抗及骨代谢的变化分析[J].海南医学院学报,2019,25(2):145-148.PANG Xiaoxia，LIAO Xiangling，WANG Minna，et al. Analysis of inflammation ,antioxidant ,insulinresistance and bone metabolism in patients withchronic periodontitis [J]. Journal of Hainan MedicalUniversity,2019,25(2):145-148.
[22] 郝瑞, 贺菲菲, 梁艳, 等. 糖尿病合并牙周炎患者龈沟液中OPG/RANKL 表达与炎症因子、自由基生成及骨代谢的相关性[J]. 海南医学院学报,2018,24(5):585-588.HAO Rui, HE Feifei, LIANG Yan, et al. The correlationof OPG/RANKL expression in gingival crevicular fluidwith inflammatory factors ,free radical generation andbone metabolism in patients with diabetes mellituscomplicated by periodontitis[J]. Journal of HainanMedical University, 2018,24 (5):585-588.

相似文献/References:

[1]姚伟莉,袁　静,耿　彪,等.慢性牙周炎患者唾液中 miR-1246的表达及其临床意义[J].现代检验医学杂志,2021,36(01):65.[doi:10.3969/j.issn.1671-7414.2021.01.017]
　YAO Wei-li,YUAN Jing,GENG Biao,et al.Expression of miR-1246 in Saliva of Patients with Chronic Periodontitis and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2021,36(05):65.[doi:10.3969/j.issn.1671-7414.2021.01.017]

备注/Memo

备注/Memo:
作者简介：祁海龙（1989-），男，本科，主治医师，专业：口腔治疗，E-mail:jiagz1980@163.com。
通讯作者：王斯路（1984-），本科，主治医师，专业：口腔颌面。E-mail:187180472@qq.com。
更新日期/Last Update: 1900-01-01