[1]邓颖云,邱成英,吴日暖,等.脓毒症患儿血浆miR-455-5p和miR-483-5p检测的临床应用[J].现代检验医学杂志,2022,37(03):79-82.[doi:10.3969/j.issn.1671-7414.2022.03.016]
　DENG Ying-yun,QIU Cheng-ying,WU Ri-nuan,et al.Clinical Application of Plasma miR-455-5p and miR-483-5p in Children with Sepsis[J].Journal of Modern Laboratory Medicine,2022,37(03):79-82.[doi:10.3969/j.issn.1671-7414.2022.03.016]
点击复制

脓毒症患儿血浆miR-455-5p和miR-483-5p检测的临床应用()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年03期
页码:
79-82
栏目:
论　著
出版日期:
2022-05-15

文章信息/Info

Title:
Clinical Application of Plasma miR-455-5p and miR-483-5p in Children with Sepsis
文章编号:
1671-7414（2022）03-079-04
作者:
邓颖云; 邱成英; 吴日暖; 匡晓玲; 卢　磊
（三亚市人民医院儿科，海南三亚572000）
Author(s):
DENG Ying-yun;  QIU Cheng-ying ;  WU Ri-nuan;  KUANG Xiao-ling;  LU Lei
（Department of Pediatrics，Sanya People’s Hospital，Hainan Sanya 572000，China）
关键词:
脓毒症; 儿童; 微小核糖核酸-455-5p; 微小核糖核酸-483-5p
分类号:
R631；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.03.016
文献标志码:
A
摘要:
目的　分析脓毒症（Sepsis）患儿血浆微小核糖核酸（micro RNA，miR）-455-5p和 miR-483-5p检测及其临床价值。方法　选择 2019年 1月～ 2020年 12月三亚市人民医院收治的 108例脓毒症患儿，根据其病情严重程度分为脓毒症非休克组（ n=65）和脓毒症休克组（ n=43）；根据脓毒症患儿 28天的生存情况，将其分为存活组（ n=74）和死亡组（ n=34），另选取 50例健康儿童作为对照组，检测各组血浆 miR-455-5p和 miR-483-5p表达水平。应用受试者工作特征曲线（ ROC）分析血浆 miR-455-5p，miR-483-5p及降钙素原（ procalcitonin，PCT）水平对脓毒症诊断及预后评估的价值。结果　脓毒症休克组血浆 miR-455-5p（4.06±1.35），miR-483-5p（3.40±1.13 ）及 PCT（19.97±9.12μg/L）水平均明显高于脓毒症非休克组（ 1.95±0.81，1.38±0.57，5.73±2.36μg/L）和对照组（ 0.78±0.24，0.35±0.10，0.02±0.01μg/L），差异均有统计学意义（ t=12.985～ 24.638，均 P＜ 0.01）。死亡组血浆 miR-455-5p（4.30±1.52），miR-483-5p（3.61±1.24）及 PCT（21.35±9.58）水平均明显高于存活组（ 1.74±0.68，1.20±0.45，4.36±1.94μg/L）和对照组，差异均有统计学意义（ t =10.513～ 21.719，均 P＜ 0.01）。ROC曲线分析显示， miR-455-5p，miR-483-5p及 PCT联合诊断脓毒症的曲线下面积（ AUC）最大（ 0.904，95%CI：0.846～ 0.967），其敏感度和特异度分别为 92.4%和 83.7%。miR-455-5p， miR-483-5p及 PCT联合预测脓毒症患儿死亡的 AUC最大（ 0.928，95%CI：0.870～ 0.984），其敏感度和特异度分别为 94.7%和 86.5%。结论　脓毒症患儿血浆 miR-455-5p和 miR-483-5p表达水平明显升高，联合 PCT检测有助于提高儿童脓毒症的诊断及预后评估价值。
Abstract:
Objective　To analyze the detection and clinical value of plasma miR-455-5p and miR-483-5p in children with Sepsis. Methods　108 cases children with sepsis admitted to Sanya People’s Hospital from January 2019 to December 2020 were divided into sepsis non shock group (n=65) and sepsis shock group (n=43). According to the 28 day survival of children with sepsis, they were divided into two groups: survival group (n=74) and death group (n=34). Another 50 healthy children were selected as the control group. The expression levels of plasma miR-455-5p and miR-483-5p were detected in each group. Receiver operating characteristic curve (ROC) was used to analyze the value of plasma levels of miR-455-5p，miR-483-5p and procalcitonin (PCT) for the diagnosis and prognosis of sepsis. Results　The levels of plasma miR-455-5p（4.06±1.35）， miR-483-5p（3.40±1.13） and PCT（19.97±9.12μg/L） in septic shock group were significantly higher than those in septic non shock group （1.95±0.81，1.38±0.57，5.73±2.36μg/L）and control group（0.78±0.24，0.35±0.10，0.02±0.01μg/ L），the differences were statistically significant (t =12.985 ～ 24.638，all P ＜ 0.01). The levels of plasma miR-455-5p （4.30±1.52），miR-483-5p（3.61±1.24 ） and PCT（21.35±9.58μg/L） in the death group were significantly higher than those in the survival group（1.74±0.68，1.20±0.45，4.36±1.94μg/L） and control group，the differences were statistically significant (t=10.513 ～ 21.719，all P ＜ 0.01). ROC curve analysis showed that the area under the curve (AUC) of miR-455- 5p, miR-483-5p and PCT in the diagnosis of sepsis was the largest (0.904, 95% CI:0.846 ～ 0.967), with a sensitivity of 92.4% and a specificity of 83.7%.The AUC of miR-455-5p, miR-483-5p and PCT in predicting the death of children with sepsis was the largest (0.928, 95% CI: 0.870 ～ 0.984), with a sensitivity of 94.7% and a specificity of 86.5%. Conclusion　The expression levels of plasma miR-455-5p and miR-483-5p in children with sepsis were significantly increased. Combined with PCT detection, it is helpful to improve the value of diagnosis and prognosis evaluation of children with sepsis.

参考文献/References:

[1] SEHGAL M, LADD H J, TOTAPALLY B. Trends in epidemiology and microbiology of severe sepsis and septic shock in children[J]. Hospital pediatrics, 2020, 10(12): 1021-1030.
[2] LINK F, KROHN K, BURGDORFF A M, et al. Sepsis diagnostics: intensive care scoring systems superior to microRNA biomarker testing[J]. Diagnostics (Basel, Switzerland), 2020, 10(9): 701.
[3] MANETTI A C, MAIESE A, PAOLO M D, et al. MicroRNAs and sepsis-induced cardiac dysfunction: a systematic review[J]. International Journal of Molecular Sciences, 2020, 22(1): 321.
[4] MAO Yueyan, SU Chang, FANG Chengchao, et al. Clinical significance of the serum miR-455- 5p expression in patients with neonatal sepsis[J]. Bioengineered, 2021, 12(1): 4174-4182.
[5] LENG Chenghui, SUN Junli, XIN Keke, et al. High expression of miR-483-5p aggravates sepsis-induced acute lung injury[J]. The Journal of Toxicological Sciences, 2020, 45(2): 77-86.
[6] YUNUS I, FASIH A, WANG Yanzhi. The use of procalcitonin in the determination of severity of sepsis, patient outcomes and infection characteristics[J]. PLoS One, 2018, 13(11): e0206527.
[7] 中华医学会儿科学分会急救学组. 中华医学会急诊 医学分会儿科学组, 中国医师协会儿童重症医师分 会. 儿童脓毒性休克( 感染性休克) 诊治专家共识 (2015 版)[J]. 中华儿科杂志, 2015, 53(8): 576-580. The Subspecialty Group of Emergency Medicine, the Society of Pediatrics, Chinese Medical Association, the Subspecialty Group of Pediatrics, The Society of Emergency Medicine, Chinese Medical Association, The Society of Pediatric Critical Care, Chinese Medical Doctor Association. Expert consensus on the diagnosis and treatment of children’s septic shock (septic shock) (2015 Edition) [J]. Chinese Journal of Pediatrics, 2015, 53 (8): 576-580.
[8] SZIL?GYI B, FEJES Z, P?CSI M, et al. Role of sepsis modulated circulating microRNAs[J]. EJIFCC, 2019, 30(2): 128-145.
[9] CHEN Pan, MIAO Ying, YAN Pujun, et al. MiR- 455-5p ameliorates HG-induced apoptosis, oxidative stress and inflammatory via targeting SOCS3 in retinal pigment epithelial cells[J]. Journal of Cellular Physiology, 2019, 234(12): 21915-21924.
[10] WANG Xuegang, ZHU Yongwu, WANG Tao, et al. MiR-483-5p downregulation contributed to cell proliferation, metastasis, and inflammation of clear cell renal cell carcinoma[J]. The Kaohsiung Journal of Medical Sciences, 2021, 37(3): 192-199.
[11] SHARMA S, UPPAL B, MANCHANDA V, et al. Diagnostic utility of procalcitonin as biomarker of sepsis in children[J]. Infect Dis (Lond), 2018, 50(7):567-568.
[12] 王军宇, 王宏伟, 刘温馨, 等. 降钙素原和血乳酸及 病情严重程度评分对脓毒症患者预后的评估价值 [J]. 中华危重病急救医学,2019, 31(8): 938-941. WANG Junyu, WANG Hongwei, LIU Wenxin, et al. Assessment values of procalcitonin, lactic acid, and disease severity scores in patients with sepsis [J]. Chinese Critical Care Medicine, 2019, 31 (8): 938-941.
[13] 叶荣宗. 外泌体miRNA 在脓毒症中的研究进展[J]. 海南医学, 2021, 32(12):1596-1599. YE Rongzong. Exosome-derived miRNAs in sepsis [J]. Hainan Medical Journal, 2021, 32 (12): 1596-1599.
[14] ZHAO Danna, LI Shilei, CUI Jie, et al. Plasma miR‐125a and miR‐125b in sepsis: Correlation with disease risk, inflammation, severity, and prognosis[J]. Journal of Clinical Laboratory Analysis, 2020, 34(2): e23036.
[15] KARAM R A, HE Zidan, KARAM N, et al. Diagnostic and prognostic significance of serum miRNA- 146-a expression in Egyptian children with sepsis in a pediatric intensive care unit[J]. Journal of Gene Medicine, 2019, 21(11): e3128.
[16] RAHMEL T, SCH?FER S T, FREY U H, et al. Increased circulating microRNA-122 is a biomarker for discrimination and risk stratification in patients defined by sepsis-3 criteria[J]. PLoS One, 2018, 13(5): e0197637.
[17] 中华医学会儿科学分会医院感染管理与控制专业委 员会. 血清降钙素原检测在儿童感染性疾病中的临床 应用专家共识[J]. 中华儿科杂志，2019，57(1) ：9-15. The Special Committee of Hospital Infection Management and Control/the Society of Pediatrics， Chinese Medical Association. Expert consensus on clinical application of serum procalcitonin in pediatric infectious disease [J]. Chinese Journal of Pediatrics, 2019, 57 (1): 9-15.

相似文献/References:

[1]安 娜,刘运双,陈 曦,等.儿童肺炎支原体感染与血清维生素D水平的相关研究[J].现代检验医学杂志,2016,31(02):117.[doi:10.3969/j.issn.1671-7414.2016.02.035]
　AN Na,LIU Yun-shuang,CHEN Xi,et al.Relationship of the Mycoplasma Pneumonia Infection and Vitamin D levels in Children[J].Journal of Modern Laboratory Medicine,2016,31(03):117.[doi:10.3969/j.issn.1671-7414.2016.02.035]
[2]张月芳a,马芳莲,朱亚宁b,等.儿童抗幽门螺旋杆菌感染治疗对肠道菌群影响的研究[J].现代检验医学杂志,2015,30(05):32.[doi:10.3969/j.issn.1671-7414.2015.05.010]
　ZHANG Yue-fanga,MA Fang-lian,ZHU Ya-ningb,et al.Research on the Effects of Anti Helicobacter Pylori Infection on Intestinal Flora of Children[J].Journal of Modern Laboratory Medicine,2015,30(03):32.[doi:10.3969/j.issn.1671-7414.2015.05.010]
[3]唐玉蓉a,王际亮a,孙婷婷a,等.儿童特发性血小板减少性紫癜Th亚群细胞因子的测定及意义[J].现代检验医学杂志,2015,30(05):143.[doi:10.3969/j.issn.1671-7414.2015.05.045]
　TANG Yu-ronga,WANG Ji-lianga,SUN Ting-tinga,et al.Determination and Clinical Significance of Th Subsets Cytokines in ITP of Children[J].Journal of Modern Laboratory Medicine,2015,30(03):143.[doi:10.3969/j.issn.1671-7414.2015.05.045]
[4]吉翔,华芸,张学军.哮喘患儿血清白介素与免疫球蛋白检测的临床意义[J].现代检验医学杂志,2015,30(06):127.[doi:10.3969/j.issn.1671-7414.2015.06.039]
　JI Xiang,HUA Yun,ZHANG Xue-jun.Clinical Significance of Combined Detection of Serum Interleukin and Immune Globulin in Children with Asthma[J].Journal of Modern Laboratory Medicine,2015,30(03):127.[doi:10.3969/j.issn.1671-7414.2015.06.039]
[5]王 玲,尹春燕,肖延风,等.噬血细胞综合征(HPS)的早期鉴别诊断和实验室结果分析[J].现代检验医学杂志,2016,31(04):107.[doi:10.3969/j.issn.16717-414.2016.04.030]
　WANG Ling,YIN Chun-yan,XIAO Yan-feng,et al.Analysis of the Etiology and Clinical Features of Bone Marrow in Children with Hemophagocytic Phenomenon[J].Journal of Modern Laboratory Medicine,2016,31(03):107.[doi:10.3969/j.issn.16717-414.2016.04.030]
[6]黄海林,王海平,张 倩,等.昆明地区儿童脑脊液细菌培养阳性结果分析[J].现代检验医学杂志,2016,31(04):151.[doi:10.3969/j.issn.1671-7414.2016.04.044]
　HUANG Hai-lin,WANG Hai-ping,ZHANG Qian,et al.Analysis of Positive Culture of Cerebrospinal Fluid in Children of Kunming Area[J].Journal of Modern Laboratory Medicine,2016,31(03):151.[doi:10.3969/j.issn.1671-7414.2016.04.044]
[7]肖海军,殷晓晴,张慧莲,等.深圳地区儿童A群致病性链球菌感染流行现状及M蛋白基因分型分析[J].现代检验医学杂志,2016,31(06):51.[doi:10.3969/j.issn.1671-7414.2016.06.014]
　XIAO Hai-jun,YIN Xiao-qing,ZHANG Hui-lian,et al.Analysis of the Prevalence Status and M Protein Gene Classification of A Group Pathogenic Streptococcus Infection of Children in Shenzhen Area[J].Journal of Modern Laboratory Medicine,2016,31(03):51.[doi:10.3969/j.issn.1671-7414.2016.06.014]
[8]招湛静,徐威力,姚雪淦.深圳市大鹏新区儿童小细胞低色素性贫血流行现状及病因探讨[J].现代检验医学杂志,2016,31(06):98.[doi:10.3969/j.issn.1671-7414.2016.06.028]
　ZHAO Zhan-jing,XU Wei-li,YAO Xue-gan.Epidemic Situation and Causes of Children of Cellule Low Pigment Anemia in Shenzhen Dapeng New District[J].Journal of Modern Laboratory Medicine,2016,31(03):98.[doi:10.3969/j.issn.1671-7414.2016.06.028]
[9]沈丽娟a,孙 杰a,吴 晓b,等.老年脓毒症患者血清清蛋白水平与其危重程度及预后的相关性[J].现代检验医学杂志,2017,32(01):131.[doi:10.3969/j.issn.1671-7414.2017.01.036]
　SHEN Li-juana,SUN Jiea,WU Xiaob,et al.Relationship of Albumin Levels with the Prognosis and Severity of Illness among Elderly Sepsis Patients[J].Journal of Modern Laboratory Medicine,2017,32(03):131.[doi:10.3969/j.issn.1671-7414.2017.01.036]
[10]招湛静,刘爱胜,刘爱玲,等.深圳地区0～7岁儿童微量元素含量现状调查[J].现代检验医学杂志,2017,32(03):149.[doi:10.3969/j.issn.1671-7414.2017.03.042]
　ZHAO Zhan-jing,LIU Ai-sheng,LIU Ai-ling,et al.Present Situation Investigation of Trace Elements of 0~7 Years Old Children in Shenzhen District[J].Journal of Modern Laboratory Medicine,2017,32(03):149.[doi:10.3969/j.issn.1671-7414.2017.03.042]
[11]洪　莉,郑　青,陈永香,等.脓毒症并发急性肾损伤患儿血清miR-452-3p的水平表达及其临床意义[J].现代检验医学杂志,2021,36(06):176.[doi:10.3969/j.issn.1671-7414.2021.06.038]
　HONG Li,ZHENG Qing,CHEN Yong-xiang,et al.Expression and Clinical Significance of Serum miR-452-3p in Children with Sepsis Complicated with Acute Kidney Injury[J].Journal of Modern Laboratory Medicine,2021,36(03):176.[doi:10.3969/j.issn.1671-7414.2021.06.038]

备注/Memo

备注/Memo:
作者简介：邓颖云（1984-）, 女，本科，主治医师，主要从事临床儿科疾病研究，E-mail：deng1589@163.com。
更新日期/Last Update: 1900-01-01