[1]曹修娥,张倡誌,鲍国林,等.山东济南地区健康成人血清超氧化物歧化酶参考区间的初步建立与验证[J].现代检验医学杂志,2023,38(06):170-173+199.[doi:10.3969/j.issn.1671-7414.2023.06.031]
　CAO Xiue,ZHANG Changzhi,BAO Guolin,et al.Establishment and Verification of Reference Interval of Serum SOD in Healthy Adults in Jinan Area of Shandong[J].Journal of Modern Laboratory Medicine,2023,38(06):170-173+199.[doi:10.3969/j.issn.1671-7414.2023.06.031]
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山东济南地区健康成人血清超氧化物歧化酶参考区间的初步建立与验证()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年06期
页码:
170-173+199
栏目:
检验与临床
出版日期:
2023-11-15

文章信息/Info

Title:
Establishment and Verification of Reference Interval of Serum SOD in Healthy Adults in Jinan Area of Shandong
文章编号:
1671-7414（2023）06-170-05
作者:
曹修娥; 张倡誌; 鲍国林; 赵士玉; 刘义庆
（山东第一医科大学附属省立医院临床医学检验部，济南 250021）
Author(s):
CAO Xiu’e;  ZHANG Changzhi;  BAO Guolin;  ZHAO Shiyu;  LIU Yiqing
（Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China）
关键词:
济南地区; 健康成人; 超氧化物歧化酶; 参考区间
分类号:
R446.11
DOI:
10.3969/j.issn.1671-7414.2023.06.031
文献标志码:
A
摘要:
目的 建立和验证山东济南地区健康成人血清超氧化物歧化酶（superoxide dismutase，SOD）的参考区间。方法 选取2020 年3 月～ 2021 年10 月在山东第一医科大学附属省立医院健康体检中心体检的健康成人为研究对象，最终共纳入2 559 例，其中男性1 293 例，女性1 266 例，收集其血清标本，采用比色法检测血清SOD 浓度水平，依据美国临床和实验室标准协会（CLSI）C28-A2 的要求，以非参数法建立基于性别和年龄的血清SOD 参考区间，并对参考区间进行验证。结果 男性血清SOD[181.30（172.00，189.45）U/ml] 高于女性[175.70（165.60，184.33）U/ml]，差异有统计学意义（Z=-9.67，P=0.000）。按年龄分组：血清SOD 水平随着年龄的增加而降低，其中男性年龄组间比较Z=-4.993 ～ -2.094，P= 0.000 ～ 0.036，女性年龄组间比较，41~50 岁与51~60 岁两年龄组间比较Z=-0.209，P=0.835，其余年龄组间比较Z=-3.847 ～ -2.034，P= 0.000 ～ 0.042。51 ～ 60 岁组血清SOD 在男、女性间的差异无统计学意义（Z=-0.526，P=0.599），其余年龄组间男性血清SOD 水平均高于女性，差异有统计学意义（Z= -7.601 ～-1.984，P= 0.000 ～ 0.047）。济南地区男性年龄按18 ～ 30，31 ～ 40，41 ～ 50，51 ～ 60 和≥ 61 岁分为5 组，参考区间分别为147.94 ～ 206.93，153.44 ～ 206.21，147.92 ～ 205.77，145.77 ～ 199.84 和140.93 ～ 200.85 U/ml； 女性年龄按18 ～ 30，31 ～ 40，41 ～ 60 和≥ 61 岁分为4 组，参考区间分别为147.17 ～ 202.31，140.76 ～ 201.59，139.85 ～ 197.64 和135.78 ～ 190.34 U/ml。各组所建立的参考区间全部通过验证。结论 初步建立了山东济南地区健康成人血清SOD 的参考区间，且血清SOD 水平存在性别和年龄差异，更好的为SOD 的临床应用提供依据。
Abstract:
Objective To establish and verify the reference interval of superoxide dismutase (SOD) for healthy adults in Jinan area. Methods A total of 2 559 healthy adults were enrolled in Shangdong Provincial Hospital Affiliated to the First Medical University of Shangdong from March 2020 to October 2021, including 1 293 males and 1 266 females. The serum samples were collected and the serum SOD levels were detected by colorimetric method, and the sex- and age-related reference intervals were determined according to the Clinical and Laboratory Standards Institute (CLSI) C28-A2 document, and the reference interval was verified. Results Serum SOD level in males was 181.30(172.00, 189.45)U/ml, which were higher than that in females 175.70(165.60, 184.33)U/ml, and the difference was statistically significant(Z=-9.67, P=0.000). Serum SOD levels decreased with the increase of age, which was compared among male groups(Z= -4.993 ～ -2.094, P= 0.000 ～ 0.036), female groups 41 ~50 years old and 51~60 years old （Z= -0.209, P=0.835） and among othe age groups(Z= -3.847 ～ -2.034, P= 0.000 ～ 0.042). There was no statistically significant difference in serum SOD between male and female in 51 ～ 60 years old group(Z=-0.526, P=0.599), and the serum SOD level of males were higher than that of females in other age groups, and the differences were statistically significant(Z= -7.601 ～ -1.984, P= 0.000 ～ 0.047). Men in Jinan were divided into 5 groups according to their ages of 18 ～ 30, 31 ～ 40, 41 ～ 50, 51 ～ 60 and ≥ 61 year old, and the reference interval was 147.94 ～ 206.93, 153.44 ～ 206.21, 147.92 ～ 205.77, 145.77 ～ 199.84 and 140.93 ～ 200.85 U/ml, respectively. Female ages were divided into 4 groups according to 18 ～ 30, 31 ～ 40, 41 ～ 60 and ≥ 61 year old, and the reference interval was 147.17 ～ 202.31, 140.76 ～ 201.59,139.85 ～ 197.64 and 135.78 ～ 190.34 U/ml, respectively. All the reference intervals had passed the verification. Conclusion The reference intervals for serum SOD of healthy adults in Jinan was initially established, and there were gender and age differences in serum SOD levels, which provides a reference for the clinical application of serum SOD.

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备注/Memo

备注/Memo:
基金项目： 山东省自然科学基金(ZR2020MH316）：LncRNA MALAT1/miR-127 通过调控WNT/B-CATENIN 通路从而影响肝细胞癌进展的机制研究；Tim-3 参与HBC 蛋白抑制慢性乙型肝炎CD8+ 细胞功能研究（ZR2016HM52）。
作者简介：曹修娥（1988-），女，硕士研究生，主管技师，主要从事临床检验及质量控制工作，E-mail：695704701@qq.com。
通讯作者：刘义庆（1979-），男，博士，副主任医师，主要从事临床检验的基础与临床研究，E-mail：yqliu1979@163.com。
更新日期/Last Update: 2023-11-15