[1]王雪怡,周婷婷,武文凤.肺结核患者ERRB基因rs12437118多态性与血清25(OH)D水平的交互作用对结核耐多药的影响研究[J].现代检验医学杂志,2025,40(06):56-61.[doi:10.3969/j.issn.1671-7414.2025.06.011]
　WANG Xueyi,ZHOU Tingting,WU Wenfeng.Interaction between rs12437118 Polymorphism of ERRB Gene and Serum 25(OH)D Level on Multi-drug Resistance of Tuberculosis in Patients with Pulmonary Tuberculosis[J].Journal of Modern Laboratory Medicine,2025,40(06):56-61.[doi:10.3969/j.issn.1671-7414.2025.06.011]
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肺结核患者ERRB基因rs12437118多态性与血清25(OH)D水平的交互作用对结核耐多药的影响研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年06期
页码:
56-61
栏目:
论著
出版日期:
2025-11-15

文章信息/Info

Title:
Interaction between rs12437118 Polymorphism of ERRB Gene and Serum 25(OH)D Level on Multi-drug Resistance of Tuberculosis in Patients with Pulmonary Tuberculosis
文章编号:
1671-7414（2025）06-056-06
作者:
王雪怡，周婷婷，武文凤
新疆维吾尔自治区人民医院，乌鲁木齐 830001
Author(s):
WANG Xueyi，ZHOU Tingting，WU Wenfeng
People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
关键词:
雌激素相关受体β; 基因多态性; 25-羟维生素D; 肺结核; 耐多药结核病
分类号:
R521；Q786
DOI:
10.3969/j.issn.1671-7414.2025.06.011
文献标志码:
A
摘要:
目的探究肺结核患者雌激素相关受体β（ERRB）基因rs12437118多态性与血清25-羟维生素D[25(OH)D]水平的交互作用及其对结核耐多药的影响。方法选取2018年1月～2023年12月新疆维吾尔自治区人民医院收治的104例耐多药结核病（MDR-TB）患者为耐多药组，另选取同期于新疆维吾尔自治区人民医院治疗的非MDR-TB患者102例为对照组，比较两组患者基因频率。采用多因素Logistic回归分析耐多药的影响因素，采用多元线性回归分析ERRB基因rs12437118多态性与耐多药及25(OH)D的关联；采用非条件Logistic回归模型分析ERRB基因rs12437118基因型与25(OH)D对耐多药的交互作用。结果耐多药组GG（50.96%），AA（13.46%）基因频率显著高于对照组（37.25%，9.80%），差异具有统计学意义（χ2=6.296，P<0.05）。两组之间G，A两种等位基因频率差异无统计学意义（χ2=1.163，P>0.05）。多因素Logistic回归分析结果显示，年龄、居住地、治疗史、吸烟史、空洞数、规律用药、职业、rs12437118等位基因G及25(OH)D均是影响患者耐多药的影响因素（Waldχ2=0.873～3.152，均P<0.05）。多元线性回归分析结果显示，rs12437118位点与耐多药风险的关联性在等位基因模型、加性模型、显性模型和隐性模型中具有显著关联性（均P<0.05）。交互作用分析结果显示，ERRB位点rs12437118基因型G等位基因与25(OH)D对耐多药的影响在相加模型和相乘模型中存在交互作用。结论肺结核患者ERRB基因rs12437118基因型G等位基因与25(OH)D对耐多药的影响在相加模型和相乘模型中存在交互作用，两者共同存在时耐多药风险更高。
Abstract:
Objective To explore the interaction between estrogen-related receptor β (ERRB) gene rs12437118 polymorphism and serum 25-hydroxyvitamin D [25(OH)D] level on tuberculosis multidrug resistance in patients with pulmonary tuberculosis. Methods A total of 104 multidrug resistant tuberculosis (MDR-TB) patients admitted to People’s Hospital of Xinjiang Uygur Autonomous Re-gion from January 2018 to December 2023 were selected as the MDR-resistant group, and 102 non-MDR-resistant tuberculosis pa-tients treated of People’s Hospital in Xinjiang Uygur Autonomous Region during the same period were selected as the control group. The gene frequency was compared between the two groups. Multivariate Logistic regression was used to analyze the influencing factors of MDR. Multiple linear regression was used to analyze the association of ERRB gene rs12437118 polymorphism with mul-tidrug resistance and 25(OH)D. The interaction between ERRB gene rs12437118 genotype and 25(OH)D on multidrug resistance was analyzed by unconditional Logistic regression model. Results The frequencies of GG(50.96%) and AA(13.46%) genes in MDR group were significantly higher than those in control group (37.25%, 9.80%), and the difference was statistically significant (χ2=6.296, P<0.05). There was no significant difference in the frequencies of G and A alleles between the two groups (χ2=1.163, P>0.05). Mul-tivariate Logistic regression analysis showed that age, place of residence, treatment history, smoking history, empty number, regular medication, occupation, rs12437118 G allele and 25(OH)D were all independent influencing factors on multidrug resistance (Wald χ2=0.873~3.152, all P<0.05). Multiple linear regression analysis showed that rs12437118 was significantly associated with MDR risk in allele model, additive model, dominant model and recessive model (all P<0.05). The results of interaction analysis showed that the effects of rs12437118 genotype G allele and 25(OH)D on multidrug resistance were interactive in additive and multiplica-tive models. Conclusion The effects of rs12437118 genotype G allele of ERRB gene and 25(OH)D on multidrug resistance in pulmonary tuberculosis patients interacted in additive model and multiplicative model, and the risk of multidrug resistance is higher when both exist together.

参考文献/References:

[1] JOV? N, MASDEU E, BRUGUERAS S, et al. Threats and interventions during the treatment of tuberculosis in an inner-city district[J]. Archivos de Bronconeumo-logia, 2021, 57(5): 330-337.
[2] ABAD C L R, RAZONABLE R R. Multi-drug resistant and rifampin-resistant tuberculosis in transplant recip-ients[J]. Transplant Infectious Disease , 2023, 25(4):e14088.
[3] SEKHAR MIRAJ S, VYAS N, KURIAN S J, et al. Vitamin D receptor gene polymorphism and vitamin D supplementation on clinical/ treatment outcome in tuberculosis: current and future perspectives[J]. Expert Review of Anti-infective Therapy, 2022, 20(9): 1179-1186.
[4] SCH?LER-TOPRAK S, SKRZYPCZAK M, GR?ND-KER C, et al. Role of estrogen receptor β,G-protein coupled estrogen receptor and estrogen-related recep-tors in endometrial and ovarian cancer[J]. Cancers, 2023, 15(10): 2845.
[5] LI Q S, ZHENG P S. ERRB inhibits the TGFβ signal-ing pathway to drive cell proliferation in cervical can-cer[J]. Cancer Research, 2023, 83(18): 3095-3114.
[6] CARBOGNIN E, CARLINI V, PANARIELLO F, et al. ERRB guides naive pluripotent cells through the forma-tive transcriptional programme[J]. Nature Cell Biology, 2023, 25(5): 643-657.
[7] 彭晓瑞,方玉玲,胡艳松,等.维生素D补充对早产儿骨代谢及体液免疫功能的影响[J].中国药物与临床,2024,24(4):238-242. PENG X R, FANG Y L, HU Y S, et al. Effect of vita-min D supplementation on the prevention of metabolic bone disease and humoral immunefunction in prema-ture infants[J]. Chinese Remedies & Clinics, 2024, 24(4): 238-242.
[8] GALYEAN S, SYN D, SUBIH H S, et al. Improving vitamin D status in bariatric surgery subjects with monthly high-dose ergocalciferol[J]. International Jour-nal for Vitamin and Nutrition Research, 2022, 92(2):109-117.
[9] AGGELETOPOULOU I, MARANGOS M, ASSIMA-KOPOULOS S F, et al. Vitamin D and microbiome:molecular interaction in inflammatory bowel disease pathogenesis[J]. The American Journal of Pathology, 2023, 193(6): 656-668.
[10] SCHMITZ L M, KINNER A, ALTHOFF K, et al. Investigation of vitamin D2 and vitamin D3 hydrox-ylation by kutzneria albida[J]. ChemBioChem, 2021, 22(13): 2266-2274.
[11] 中华人民共和国国家卫生和计划生育委员会.肺结核诊断[J].传染病信息,2017,30(6):309-320. National Health and Family Planning Commission of the People’s Republic of China. Diagnosis of pulmo-nary tuberculosis[J]. Infectious Disease Information, 2017, 30(6): 309-320.
[12] ZHENG R J, LI Z Q, HE F S, et al. Genome-wide asso-ciation study identifies two risk loci for tuberculosis in Han Chinese[J]. Nature Communications, 2018, 9(1):4072.
[13] 段淑娟,赵玲娟,李传友,等.ERRB rs12437118多态性与中国华北汉族人群结核病易感性的关系[J].中国防痨杂志,2023,45(12):1152-1157. DUAN S J, ZHAO L J, LI C Y, et al. Association of ERRB rs12437118 polymorphisms with tuberculosis susceptibility of Han population in northern China[J]. Chinese Journal of Antituberculosis, 2023, 45(12):1152-1157.
[14] QU Y, WU Y C, JIANG H. Research progress in the pharmacological actions of the multiple effects and selectivity of the vitamin D analogue paricalcitol: a nar-rative review[J]. Annals of Palliative Medicine, 2021, 10(10): 11177-11190.
[15] 李宝花,麦伟明,黄牧,等.血清1,25-(OH)_(2)VitD3水平及VDR基因rs2228570 T/C位点多态性与肺结核患者易感性分析[J].热带医学杂志,2022,22(1):75-77, 90. LI B H, MAI W M, HUANG M, et al. Level of 1,25-(OH)2VitD3 and susceptibility of polymorphism of VDR gene rs2228570 T/C locus in patients with pul-monary tuberculosis[J]. Journal of Tropical Medicine, 2022, 22(1): 75-77, 90.
[16] PAL R, CHOUDHURY S, KUMAR H, et al. Vitamin D deficiency and genetic polymorphisms of vitamin D-as-sociated genes in Parkinson’s disease[J]. the European Journal of Neuroscience, 2023, 58(5): 3362-3377.
[17] 鹿莎,李瑞华,陈雪蓉,等.维生素D受体单核苷酸多态性与妊娠期高血压疾病易感性的相关性分析[J].中国妇幼健康研究,2024,35(3):35-42. LU S, LI R H, CHEN X R, et al. Correlation analysis of vitamin D receptor single nucleotide polymorphisms and susceptibility to hypertensive disorders of preg-nancy[J]. Chinese Journal of Woman and Child Health Research, 2024, 35(3): 35-42.
[18] JOHNSON C R, THACHER T D. Vitamin D: immune function, inflammation, infections and auto-immuni-ty[J]. Paediatrics and International Child Health, 2023, 43(4): 29-39.
[19] KNUDSEN T E, HAMILTON W B, PROKS M, et al. A bipartite function of ERRB can integrate signaling over time to balance self-renewal and differentiation[J]. Cell Systems, 2023, 14(9): 788-805.e8.
[20] ZHU A, KUZNIA S, BOAKYE D, et al. Vitamin D-binding protein, bioavailable, and free 25(OH)D,and mortality: a systematic review and Meta-analysis[J]. Nutrients, 2022, 14(19): 3894.

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备注/Memo

备注/Memo:
作者简介：王雪怡（1984-），女，本科，中级护师，研究方向：卫生事业管理，E-mail：657082317@qq.com。
通讯作者：周婷婷（1977-），女，硕士研究生，副主任医师，研究方向：劳动卫生与环境卫生学，E-mail：624521055@qq.com。
更新日期/Last Update: 2025-11-15