[1]罗 亮,许剑利,程其军,等.卵巢子宫内膜异位囊肿患者血清APRIL 与NDRG1 的水平表达及其临床价值研究[J].现代检验医学杂志,2024,39(02):124-128.[doi:10.3969/j.issn.1671-7414.2024.02.023]
　LUO Liang,XU Jianli,CHENG Qijun,et al.Expression of Serum APRIL and NDRG1 Levels in Patients with Ovarian Endometrioma and Their Clinical Value[J].Journal of Modern Laboratory Medicine,2024,39(02):124-128.[doi:10.3969/j.issn.1671-7414.2024.02.023]
点击复制

卵巢子宫内膜异位囊肿患者血清APRIL 与NDRG1 的水平表达及其临床价值研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年02期
页码:
124-128
栏目:
论著
出版日期:
2024-03-31

文章信息/Info

Title:
Expression of Serum APRIL and NDRG1 Levels in Patients with Ovarian Endometrioma and Their Clinical Value
文章编号:
1671-7414（2024）02-124-05
作者:
罗 亮; 许剑利; 程其军; 阴 莉
（自贡市第一人民医院妇产科，四川自贡 643000）
Author(s):
LUO Liang;  XU Jianli;  CHENG Qijun;  YIN Li
（Department of Obstetrics and Gynecology, Zigong First People’s Hospital, Sichuan Zigong 643000, China）
关键词:
卵巢子宫内膜异位囊肿; 增殖诱导配体; N-myc 下游调节基因1
分类号:
R711.71；R392.11
DOI:
10.3969/j.issn.1671-7414.2024.02.023
文献标志码:
A
摘要:
目的 观察血清增殖诱导配体（a proliferation inducing ligand，APRIL），N-myc 下游调节基因1（N-mycdownstream regulated gene 1，NDRG1）水平变化，并分析其对卵巢子宫内膜异位囊肿（ovarian endometriomas，OEM）的诊断价值。方法 选取2021 年7 月～ 2022 年7 月在自贡市第一人民医院就诊的132 例OEM 患者作为观察组，并进行定期随访，根据患者预后病情有无复发分为复发组（n=50）和未复发组（n=82）。同期在该院体检的健康者78 例为对照组。采用酶联免疫吸附法（enzyme linked immunosorbent assay，ELISA）检测血清中APRIL 和NDRG1 水平；并对复发组和未复发组的一般资料进行比较；采用Logistic 回归分析影响OEM 预后的相关因素；用Pearson 法分析OEM患者血清APRIL 与NDRG1 表达相关性；绘制受试者工作特征（receiver operating characteristic，ROC）曲线分析血清APRIL 和NDRG1 对OEM 的诊断价值。结果 与对照组相比，APRIL 水平（35.28 ± 6.81ng/ml vs 26.37 ± 3.19ng/ml）和NDRG1 水平（124.39 ± 15.67μg/L vs 9.67 ± 10.82μg/L）升高，差异具有统计学意义（t=10.864，17.278，均P ＜ 0.05）。与未复发组比较，复发组血清APRIL（40.38 ± 7.88ng/ml vs 32.16 ± 6.18ng/ml）和NDRG1（132.04 ± 19.83μg/L vs119.73 ± 13.16μg/L）水平升高，差异具有统计学意义（t=6.668，4.287，均P ＜ 0.05）。Logistic 回归分析显示，血清APRIL 和NDRG1 水平是影响OEM 患者预后的危险因素（Waldχ2=11.839，28.437，均P ＜ 0.001）。Pearson 法分析结果显示，OEM 患者血清APRIL 水平与NDRG1 水平呈正相关（r=0.439，P<0.001）。血清APRIL，NDRG1 水平联合诊断OEM 的曲线下面积（AUC）为0.849，灵敏度和特异度分别为73.95%，85.37%，优于APRIL 和NDRG1 单独预测（Z=2.644，2.094，P=0.008，0.036）。结论 子宫内膜异位囊肿患者血清APRIL 和NDRG1 水平升高，二者联合对子宫内膜异位囊肿诊断具有较高的临床价值，且与子宫内膜异位囊肿患者的预后密切相关。
Abstract:
Objective　To observe the changes in serum a proliferation inducing ligand (a proliferation inducing ligand, APRIL) and N-myc downstream regulated gene 1 (N-myc downstream regulated gene 1, NDRG1) levels, and analyze their diagnostic value for ovarian endometrioma (OEM). Methods　From July 2021 to July 2022, 132 patients with OEM who visited Zigong First People’s Hospital were regarded as the observation group, and regular follow-up was conducted. According to the prognosis of these patients, they were grouped into the recurrence group (n=50) and the non recurrence group (n=82). Meanwhile, 78 healthy individuals who had their medical checkups at the hospital during the same period were the control group. Enzyme linked immunosorbent assay (ELISA) was applied to detect serum APRIL and NDRG1 levels, and the general data of the recurrent and non recurrent groups were compared. Logistic regression analysis was applied to analyze the relevant factors affecting the prognosis of OEM. Pearson analysis was applied to explore the correlation between serum APRIL and NDRG1 levels in patients with OEM. Receiver operating characteristic (ROC) curve was applied to evaluate the diagnostic value of serum APRIL, NDRG1 levels and their combination for OEM. Results　Compared with the control group, APRIL level (35.28 ± 6.81ng/ml vs 26.37 ± 3.19ng/ml) and NDRG1 level (124.39 ± 15.67μg/L vs 9.67 ± 10.82μg/L) in observation group were increased, and the differences were significant (t=10.864, 17.278, all P<0.05). Compared with the non recurrence group, the serum levels of APRIL (40.38 ± 7.88ng/ml vs 32.16 ± 6.18ng/ml) and NDRG1 (132.04 ± 19.83μg/L vs 119.73 ± 13.16μg/L) in the recurrence group were increased, and the differences were significant (t=6.668, 4.287, all P<0.05). Logistic regression analysis showed that serum APRIL and NDRG1 levels were risk factors for the prognosis of patients with OEM (Waldχ2=11.839, 28.437, all P ＜ 0.001). Pearson method analysis results showed a positive correlation between serum APRIL level and NDRG1 level in patients with OEM (r=0.439, P<0.001). The area under the curve (AUC) of combined diagnosis of serum APRIL and NDRG1 levels in patients with OEM was 0.849, with a sensitivity and specificity of 73.95% and 85.37%, respectively, which was better than the single prediction of APRIL and NDRG1 (Z =2.644, 2.094, P=0.008, 0.036). Conclusion　The serum levels of APRIL and NDRG1 were increased in patients with OEM. The combination of the two has high clinical value in the diagnosis of OEM, which may be closely related to the prognosis of patients with OEM.

参考文献/References:

[1] 中国医师协会妇产科医师分会, 中华医学会妇产 科学分会子宫内膜异位症协作组. 子宫内膜异位 症诊治指南( 第三版)[J]. 中华妇产科杂志, 2021,56(12)：812-824. Chinese Obstetricians and Gynecologists Association,Cooperative Group of Endometriosis, Chinese Society of Obstetrics and Gynecology, Chinese Medical Association. Guideline for the diagnosis and treatment of endometriosis (Third edition)[J]. Chinese Journal of Obstetrics and Gynecology, 2021, 56(12): 812-824.
[2] 冷金花, 戴毅, 李晓燕. 子宫内膜异位症诊治新理念 [J]. 中华妇产科杂志, 2021, 56(12)：831-835. LENG Jinhua, DAI Yi, LI Xiaoyan. New concept of diagnosis and treatment of endometriosis[J]. Chinese Journal of Obstetrics and Gynecology, 2021, 56(12):831-835.
[3] 舒丹, 乔英琴, 彭燕, 等. 卵巢子宫内膜异位囊肿治 疗方式对卵巢功能及妊娠结局的影响[J]. 中国计划 生育学杂志, 2020, 28(4)：561-565. SHU Dan, QIAO Yingqin, PENG Yan, et al. Influence of different treatment of ovarian endometriosis cyst on ovarian function and pregnancy outcomes[J]. Chinese Journal of Family Planning, 2020, 28(4): 561-565.
[4] 薛乃瑞, 李绪清, 黄苗苗, 等. 卵巢子宫内膜异位囊 肿剥除术对冻胚移植结局的影响[J]. 安徽医科大学 学报, 2019, 54(5)：821-825. XUE Nairui, LI Xuqing, HUANG Miaomiao, et al. Effect of ovarian endometriosis cystectomy on the outcome of frozen embryo transplantation[J]. Acta Universitatis Medicinalis Anhui, 2019, 54(5): 821-825.
[5] MAGLIOZZI R, MARASTONI D, CALABRESE M. The BAFF / April system as therapeutic target in multiple sclerosis[J]. Expert Opinion on Therapeutic Targets, 2020, 24(11): 1135-1145.
[6] 潘超, 冯永恒, 朱宇, 等. APRIL 及PGR 检测指标 在胃炎、胃癌中的诊断价值分析[J]. 胃肠病学和肝 病学杂志, 2019, 28(6)：606-609. PAN Chao, FENG Yongheng, ZHU Yu, et al. Analysis of the diagnostic value of APRIL and PGR in gastritis and gastric cancer[J]. Chinese Journal of Gastroenterology and Hepatology, 2019, 28(6): 606-609.
[7] AIKEMU B, SHAO Yanfei, YANG Guang, et al. NDRG1 regulates filopodia-induced colorectal cancer invasiveness via modulating CDC42 activity[J].International Journal of Biological Sciences, 2021,17(7): 1716-1730.
[8] 荆羽萌, 赵海潮, 白丽娜, 等. NDRG1 通过上皮- 间 充质转化与子宫内膜样癌MELF 浸润模式的相关研 究[J]. 医学综述, 2020, 26(3)：491-497. JING Yumeng, ZHAO Haichao, BAI Lina, et al. Study on the relationship between the invasion pattern of the MELF and the transformation of NDRG1 through epithelial-mesenchymal[J]. Medical Recapitulate, 2020,26(3): 491-497.
[9] 中华医学会妇产科学分会子宫内膜异位症协作组. 子宫内膜异位症的诊治指南[J]. 中华妇产科杂志,2015, 56(3): 161-169. Cooperative Group of Endometriosis, Chinese Society of Obstetrics and Gynecology, Chinese Medical Association. Guideline for the diagnosis and treatment of endometriosis[J]. Chinese Journal of Obstetrics and Gynecology, 2015, 56(3): 161-169.
[10] 裘陆平, 朱卫平, 余飞成, 等. 多模态磁共振成像在 卵巢子宫内膜异位囊肿诊断中的应用观察[J]. 中国 妇幼保健, 2020, 35(1): 163-166. QIU Luping, ZHU Weiping, YU Feicheng, et al. Application of multimodal magnetic resonance imaging in diagnosis of ovarian endometriosis cyst[J]. Maternal and Child Health Care of China, 2020, 35(1): 163-166.
[11] 甘金裕, 褚伯良. 促性腺激素释放激素激动剂预处 理对卵巢子宫内膜异位囊肿腹腔镜手术患者卵巢功 能的影响[J]. 中国妇幼保健, 2020, 35(2): 243-245. GAN Jinyu, CHU Bailiang. Effect of gonadotropinreleasing hormone agonist pretreatment on ovarian function of patients with ovarian endometriosis cyst laparoscopic surgery[J]. Maternal and Child Health Care of China, 2020, 35(2): 243-245.
[12] 黄骊莉, 郭晶晶, 徐花, 等. 化瘀消癥汤保留灌肠术 后辅助治疗中重度卵巢子宫内膜异位囊肿的临床观 察[J]. 中国医师杂志, 2020, 22(9): 1407-1410. HUANG Lili, GUO Jingjing, XU Hua, et al. Clinical observation of Huayu Xiaozheng decoction retention enema for the adjuvant treatment of moderate and severe ovarian endometrioma after surgery[J]. Journal of Chinese Physician, 2020, 22(9): 1407-1410.
[13] 张汉涛, 何好超, 王国彪. 超声介入穿刺与腹腔镜手 术治疗卵巢子宫内膜异位囊肿的效果比较[J]. 哈尔 滨医药, 2020, 40(5): 424-426. ZHANG Hantao, HE Haochao, WANG Guobiao. Comparison of the effects of ultrasound interventional puncture and laparoscopic surgery for ovarian endometriotic cyst[J]. Harbin Medical Journal, 2020,40(5): 424-426.
[14] OTTOLINA J, SCHIMBERNI M, MAKIEVA S, et al. Early-life factors, in-utero exposures and endometriosis risk: a meta-analysis[J]. Reproductive Biomedicine Online, 2020, 41(2): 279-289.
[15] ANASTASIU C V, MOGA M A, ELENA NECULAU A, et al. Biomarkers for the noninvasive diagnosis of endometriosis: state of the art and future perspectives[J]. International Journal of Molecular Sciences, 2020, 21(5): 1750.
[16] CAI Wei, YANG Jule, LIU Yini, et al. Association between phthalate metabolites and risk of endometriosis: a meta-analysis[J]. International Journal of Environmental Research and Public Health, 2019,16(19): 3678.
[17] FOULADSERESHT H, ZIAEE S M, ERFANI N, et al. Serum levels of APRIL increase in patients with glioma, meningioma and schwannoma[J]. Asian Pacific Journal of Cancer Prevention, 2019, 20(3): 751-756.
[18] YANG Jia, ZHOU Jiaojiao, WANG Xin, et al. Risk factors for severe acute kidney injury among patients with rhabdomyolysis[J]. BMC Nephrology, 2020,21(1): 498.
[19] SKEDSMO F S, ESPENES A, TRANULIS M A, et al. Impaired NDRG1 functions in Schwann cells cause demyelinating neuropathy in a dog model of Charcot-Marie-Tooth type 4D[J]. Neuromuscular Disorders,2021, 31(1): 56-68.
[20] CHEKMAREV J, AZAD M G, RICHARDSON D R. The oncogenic signaling disruptor, NDRG1: molecular and cellular mechanisms of activity[J]. Cells, 2021,10(9): 2382.
[21] 张璇, 赵爱琴, 邹丹, 等. 子宫内膜异位症患者血清 miR-455 和FABP4 表达水平及临床意义[J]. 现代检 验医学杂志, 2022, 37(4): 49-52, 158. ZHANG Xuan, ZHAO Aiqin, ZOU Dan, et al. Expression level and clinical significance of serum miR-455 and FABP4 in patients with endometriosis[J].Journal of Modern Laboratory Medicine, 2022, 37(4):49-52, 158.

备注/Memo

备注/Memo:
作者简介：罗亮（1988-），男，本科，主治医师，研究方向：妇产科疾病，E-mail：fxdowhw@163.com。
通讯作者：许剑利（1971-），女，硕士，主任医师，研究方向：妇科内分泌，E-mail：xujianli@163.com。
更新日期/Last Update: 2024-03-15