[1]杨祥丽,王佃鹏,李培茂,等.阶梯式离心法血小板提取及鉴定方法的建立[J].现代检验医学杂志,2017,32(02):135-137，140.[doi:10.3969/j.issn.1671-7414.2017.02.037]
　YANG Xiang-li,WANG Dian-peng,LI Pei-mao,et al.Isolation and Identification of Platelet by Stepped Centrifugal Method[J].Journal of Modern Laboratory Medicine,2017,32(02):135-137，140.[doi:10.3969/j.issn.1671-7414.2017.02.037]
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阶梯式离心法血小板提取及鉴定方法的建立()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年02期
页码:
135-137，140
栏目:
研究简报·实验技术
出版日期:
2017-03-25

文章信息/Info

Title:
Isolation and Identification of Platelet by Stepped Centrifugal Method
文章编号:
1671-7414(2017)02-135-04
作者:
杨祥丽; 王佃鹏; 李培茂; 张志敏; 邓丽丹; 张艳芳; 周 飞; 黄先青
深圳市职业病防治院,广东深圳 518001
Author(s):
YANG Xiang-li; WANG Dian-peng; LI Pei-mao;  ZHANG Zhi-min; DENG Li-dan; ZHANG Yan-fang; ZHOU Fei; HUANG Xian-qing
Shenzhen Occupational Disease Prevention Hospital,Guangdong Shenzhen 518001,China
关键词:
血小板;  提取;  鉴定
分类号:
R446.113
DOI:
10.3969/j.issn.1671-7414.2017.02.037
文献标志码:
A
摘要:
目的 建立一种血小板提取及鉴定方法。方法选择10名健康志愿者,清晨空腹采集EDTA抗凝静脉血3管,每管2 ml,分为全血细胞管、富血小板血浆提取对照管(对照组)和阶梯式离心血小板提取实验管(实验组)。对提纯的血小板进行得率计算、显微镜下形态观察和血小板活性功能试验,并对白细胞特有HGB基因和血小板线粒体ND1基因进行定量分析。结果 对照组和实验组均提取和检测到了血小板,形态学观察发现实验组全部为血小板,无白细胞和红细胞残留; 对照组可见血小板和零星的白细胞。实验组血小板获得率显著高于对照组,HGB基因含量显著低于对照组,差异有统计学意义(t=-3.281,-2.865; P<0.05)。实验组ND1基因含量高于对照组,单个血小板活性功能吸光度值低于对照组,差异无统计学意义(t=-0.046,-0.799; P>0.05)。结论 建立了一种阶梯式离心血小板提取及鉴定方法,该方法可用于血小板基因研究和活性功能等实验分析。
Abstract:
Objective To establish a method for the isolation and identification of platelets.Methods 10 healthy volunteers were selected to collect the EDTA anticoagulant venous blood of 3 tubes,each tube was 2 ml,which was divided into the whole blood cell tube,plateletrich plasma(control group),and stepped centrifugal platelet extract(experiment group).Platelet was isolated by simple centrifugation method(PRP)and stepped centrifugal method.The two groups were full blood count and analyzed by microscopic morphology and platelet activity test.Leukocyte-specific HGB gene and platelet mitochondrial ND1 gene content was analyzed by real time PCR.Results Platelets were extracted and detected in control group andexperimental group.Platelets were found and white blood cells and red blood cells were not remained in experimental group.Platelets and sporadic white bloodcells were found in control group.The platelet pick up rate of experiment groupwas significantly higher than control group,the difference was statisticallysignificant.Experimental gene content HGB of experiment group was significantly lower than control group,the difference was statistically significant(t=-3.281,-2.865,P<0.05).ND1 gene content of experiment group higher than thecontrol group, the difference was not statistically significant.There was nosignificant difference for platelet activity test between experimental group andcontrol group(t=-0.046,-0.799,P>0.05).Conclusion A isolation and identification method of stepped centrifugal platelet wasestablished.The method can be used for the study of platelet gene and the functional analysis of platelets.

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备注/Memo

备注/Memo:
基金项目:深圳市卫生计生系统科研项目(编号:201607059),深圳市科技计划项目(编号:JCYJ20160429090813380)。
作者简介:杨祥丽(1982-),女,大学本科,主管检验师,从事临床检验工作,E-mail:lizi8332@163.com。
通讯作者:张艳芳(1970-),女,硕士,主任技师,从事临床检验研究,E-mail:13923431511@139.com。
更新日期/Last Update: 2017-04-10