[1]靳庆娥,苏建荣.北京地区汉族女性人群SYK基因启动子区-803A>Trs290987单核苷酸多态性与乳腺癌易感性分析[J].现代检验医学杂志,2018,33(02):5-7.[doi:10.3969/j.issn.1671-7414.2018.02.001]
　JIN Qing-e,SU Jian-rong.Associations of SYK Promoter-803A >T(rs290987)Single Nucleotide Polymorphisms with Susceptibility to Breast Cancer of Han Female Population in Beijing Area[J].Journal of Modern Laboratory Medicine,2018,33(02):5-7.[doi:10.3969/j.issn.1671-7414.2018.02.001]
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北京地区汉族女性人群SYK基因启动子区-803A>Trs290987单核苷酸多态性与乳腺癌易感性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年02期
页码:
5-7
栏目:
论著
出版日期:
2018-04-05

文章信息/Info

Title:
Associations of SYK Promoter-803A >T(rs290987)Single Nucleotide Polymorphisms with Susceptibility to Breast Cancer of Han Female Population in Beijing Area
文章编号:
1671-7414(2018)02-005-03
作者:
靳庆娥; 苏建荣
首都医科大学附属北京友谊医院检验科,北京 100050
Author(s):
JIN Qing-e; SU Jian-rong
Department of Clinical Laboratory, Beijing Friendship Hospital Affiliated to Capital Medical University,Beijing100050,China
关键词:
乳腺癌;  脾酪氨酸激酶基因启动子;  单核苷酸多态性
分类号:
R737.9; R730.43
DOI:
10.3969/j.issn.1671-7414.2018.02.001
文献标志码:
A
摘要:
目的 探讨脾酪氨酸激酶(spleen tyrosine kinase,SYK)基因启动子区-803A>T(rs290987)单核苷酸多态与汉族女性人群乳腺癌的相关性。方法 采用病例-对照研究,以聚合酶链反应(PCR)联合DNA直接测序法检测57例乳腺癌患者和60例与之年龄相匹配的健康对照者syk基因启动子区-803位点的基因型及等位基因频率。采用ELISA方法定量检测受试者血清SYK水平。结果 -803A>T多态T等位基因频率在乳腺癌组明显高于对照组(χ2=5.348,P=0.021)。与AA基因型相比(TA+TT)基因型可显著增加乳腺癌发病风险,OR值为2.87,95% CI=1.27～6.49。乳腺癌组血清SYK水平显著低于对照组(F=33.278,P<0.01)。结论北京地区汉族女性人群SYK基因-803位点基因型TA和TT是乳腺癌发病的危险因素。
Abstract:
Abstract:Objective To evaluate the association of the SYK gene promoter -803A/T polymorphism and breast cancer susceptibility during Han descent female population in Beijing area.Methods In this case control study,genotype of SYK(A>T at -803,rs290987)was determinedby polymerase chain reaction combined with DNA direct sequencing in blood samples of 57 breast cancer cases and 60 age-matched healthy controls.They were all female.Genotype and allele frequency distributions of -803A/T were determined and analyzed.ELISA test was performed to detect the serum level of SYK in thetwo groups.Results The frequencies of the T allele andTA+TT genotypes of the -803A>T were found to be significantly higher in the breast cancer patients in contrast to the healthy individuals of the control group(χ2=5.348,P=0.021).Patients with the T allele(TA+TT)had a 2.87-fold risk of developing breast cancer compared with those with the A allele(AA)(OR=2.87; 95% CI=1.27～6.49).The test group had significantly decreased levelof SYK in serum compared to normal controls(F=33.278,P<0.01).Conclusion This study revealed that the genotype TA and TT of theSYK gene -803 loci of Han female population in the Beijing area are the riskfactors for the breast cancer.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:首都医科大学基础-临床科研合作基金(编号:12JL35)。 作者简介:靳庆娥(1981-),女,博士,助理研究员,主要从事病原体分子诊断工作,E-mail:jinqinge@163.com。 通讯作者:苏建荣,女,主任医师,博士生导师,主要从事感染性疾病诊断方法及病原微生物耐药机制研究,E-mail:youyilab@163.com。
更新日期/Last Update: 2018-04-16