[1]高建华,何颖辉.冠心病并发幽门螺杆菌感染患者血清HP抗体谱特征与炎性因子水平相关性分析[J].现代检验医学杂志,2019,34(04):53-57.[doi:10.3969/j.issn.1671-7414.2019.04.013]
　GAO Jian-hua,HE Ying-hui.Correlation Analysis of Serum Antibody Spectrum and Inflammatory Factor Level in Patients with Coronary Heart Disease Complicated with Helicobacter Polori Infection[J].Journal of Modern Laboratory Medicine,2019,34(04):53-57.[doi:10.3969/j.issn.1671-7414.2019.04.013]
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冠心病并发幽门螺杆菌感染患者血清HP抗体谱特征与炎性因子水平相关性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年04期
页码:
53-57
栏目:
论著
出版日期:
2019-08-20

文章信息/Info

Title:
Correlation Analysis of Serum Antibody Spectrum and Inflammatory Factor Level in Patients with Coronary Heart Disease Complicated with Helicobacter Polori Infection
文章编号:
1671-7414(2019)04-053-05
作者:
高建华; 何颖辉
(杭州市西湖区中西医结合医院,杭州310000)
Author(s):
GAO Jian-hua; HE Ying-hui
(Xihu District Hospital of Traditional Chinese and Western Medicine in Hangzhou City,Hangzhou 310000,China)
关键词:
冠心病;  幽门螺杆菌;  血清幽门螺杆菌抗体;  血清抗体谱
分类号:
R541.4; R392.11
DOI:
10.3969/j.issn.1671-7414.2019.04.013
文献标志码:
A
摘要:
目的 探讨冠心病(CHD)并发幽门螺杆菌(HP)感染者血清炎性因子水平和HP抗体谱特征。方法 收集2016年12月～2017年12月来院就诊的CHD和健康体检者的血清样本524例,分别设为CHD组(疾病组)296例与非CHD组(对照组)228例,采用血清HP抗体进行检测,筛选CHD并发HP感染患者,另采用化学发光法和免疫印迹法对相应的炎性因子和HP血清抗体谱进行检测,比较两组人群各指标间的差异性。结果 在两组人群中疾病组的HP抗体阳性率(52.03%,154/296)高于对照组(35.09%,80/228),差异具有统计学意义(χ2=14.95,P<0.05),疾病组各炎性因子含量均高于对照组,两组间差异均具有统计学意义(t=-33.34～19.96,均P<0.05); 疾病组并发HP感染与对照组的抗体谱分析发现,VacA-95KD,VacA-91KD,UreA-66KD和UreB-30KD抗体在两组间的阳性率差异均无统计学意义(χ2=0.22～3.49,均P>0.05),而疾病组并发HP感染患者血清CagA-116KD抗体阳性率(76.62%,118/154)高于对照组中HP感染者(62.5%,50/80),且两组间的差异具有统计学意义(χ2=5.19,P<0.05),疾病组中并发HP感染者血清I型阳性率(76.62%,118/154)高于对照组并发HP感染者(62.5%,50/80),两组间差异具有统计学意义(χ2=5.19,P<0.05)。疾病组按照HP感染血清型间及与非感染患者血清因子间比较发现,I型血清型血清超敏C反应蛋白(hs-CRP)、同型半胱氨酸(Hcy)、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的含量高于II型血清型,且II型血清型hs-CRP,TNF-α,IL-6和Hcy的含量高于非HP感染CHD患者,各组间差异均具有统计学意义(F=524.8～2 829.29,均P<0.05)。结论该文对于CHD患者并发HP感染的血清学特征进行调查,验证了CHD发生于HP感染之间的关联性,积累了HP感染的流行病学资料,为进一步研究CHD的发生、发展以及治疗奠定了科学依据。
Abstract:
Objective To investigate the levels of serum inflammatory factors and the characteristics of HP antibody spectrum in patients with coronary heart disease(CHD)complicated with Helicobacter pylori(HP).Methods 524 serum samples were collected from CHD patients and health examinees from December 2016 to December 2017,296 in CHD group and228 in non-CHD group.Serum HP antibody was used to screen patients with CHD complicated with HP infection.The corresponding inflammatory factors and HP serum antibody spectrum were detected by chemiluminescence and immunoblotting,respectively.The differences of two groups were compared among population indices.Results The positive rate of HP antibody in the two groups(52.03%,154/296)was higher than that in the control group(35.09%,80/228).The difference was statistically significant(χ2=14.95,P<0.05).Thecontent of inflammatory factors in the disease group was higher than that in thecontrol group, and the difference was statistically significant(t=-33.34～-19.96,all P< 0.05).The antibody analysis of patients of HP infectionin the disease group and the control group showed no statistically significant difference in the positive rate of VacA-95KD,VacA-9 KD,UreA-66KD and UrteB-30KD antboy between the two groups(χ2=0.22～3.49,all P<0.05).The positive rates of serum CagA-116KD antibodies in patients with HP infection in the disease group(76.62%,118/154)were higher than those in the control group(62.5%,50/80),and the difference between the two groups was statisticallysignificant(χ2=5.19,P<0.05).The positive rates of serum type I antibodies in patients with HP infection in the disease group(76.62%,118/154)werehigher than those in the control group(62.5%,50/80).The difference betweenthe two groups was statistically significant(χ2=5.19,P<0.05).118/154were higher than those in the control group(62.5%,50/80).There was a significant difference between the two groups(χ2=5.19,P<0.05).According tothe comparison between serotypes of HP infection and serum factors of non-HP infected patients,the levels of serum type I hypersensitive C-reactive protein(hs-CRP),homocysteine(Hcy),tumor necrosis factor-alpha(TNF-alpha)and interleukin-6(IL-6)were higher than those of type II serotype,and the levels of serum type II Hs-CRP,TNF-alpha,IL-6 and H cysteine were higher than thoseof non-HP infected CHD patients.The difference was statistically significant(F=524.8～2829.29,all P<0.05).Conclusion Theserological characteristics of CHD patients complicated with HP infection wereinvestigated in this paper.The correlation between the occurrence of CHD and HPinfection was verified.The epidemiological data of HP infection were accumulated,which laid a scientific basis for further research on the occurrence,development and treatment of CHD.

参考文献/References:

[1] 柴晓芮,崔凯,叶中倪.高血压患者发生冠心病的相关危险因素分析[J].中华老年心脑血管病杂志,2016,18(6):590-592. CHAI Xiaorui,CUI Kai,YE Zhongni.Risk factors for coronary heart disease in hypertensive patients[J].Chin J Geriatr Heart Brain Ves Dis,2016,18(6):590-592. [2] 田杰,卢建刚,曾秋蓉,等.同型半胱氨酸和颈动脉内膜中层厚度与老年冠心病严重程度的相关性[J].中华老年心脑血管病杂志,2015,17(3):266-269. TIAN Jie,LU Jiangang,ZENG Qiurong,et al.Association of serum homocysteine level and carotid IMT with coronary heart disease in elderly subjects[J].Chin J Geriatr Heart Brain Ves Dis,2015,17(3):266-269. [3] 王成,刘艳宾,韩明磊.幽门螺杆菌感染与冠心病发病的相关性研究[J].中华医院感染学杂志,2014,24(14):3526-3527,3530. WANG Cheng,LIU Yanbin,HAN Minglei.Research on correlation betweenHelicobacter pylori infection and coronary heat disease[J].Chin J Nosocomiol,2014,24(14):3526-3527,3530. [4] 吕仲兰,王永红,张爱民.冠心病患者幽门螺杆菌感染与血清同型半胱氨酸水平的关系研究[J].中华医院感染学杂志,2014,24(23):5786-5788. L(¨overU)Zhonglan,WANG Yonghong,ZHANG Aimin.Study on relationship of Helicobacter pylori infection in patients with coronary heart diseaseand serum homocysteine levels[J].Chin J Nosocomiol,2014,24(23):5786-5788. [5] 刘杨,刘学芳,吴永昌,等.冠心病患者幽门螺杆菌感染及患病相关因素的临床研究[J].现代检验医学杂志,2013,28(6):18-21. LIU Yang,LIU Xuefang,WU Yongchang,et al.Research on the correlation of Helicobacter pylori infection and invasion correlation factor in patients with coronary heart disease[J].J Mod Lab Med,2013,28(6):18-21. [6] 刘贵生,李文生,郭雪艳,等.不同亚型肠上皮化生和胃癌中CDX2蛋白的表达及其与幽门螺杆菌感染的关系[J].现代检验医学杂志,2017,32(2):10-14. LIU Guisheng,LI Wensheng,GUO Xueyan,et al.Expression of CDX2 andits relationship with Helicobacter pylori infection in different subtypes of intestinal metaplasia and gastric cancer[J].J Mod Lab Med,2017,32(2):10-14. [7] 周丽雅,宋志强.幽门螺杆菌感染与胃癌[J].中华内科杂志,2014,53(5):349-351. ZHOU Liya,SONG Zhiqiang.Helicobacter pylori infection and gastric cancer[J].Chin J Intern Med,2014,53(5):349-351. [8] 张丽,张保朝,王璐,等.冠状动脉粥样硬化性心脏病患者细胞毒素相关蛋白基因A幽门螺杆菌感染状况研究[J].中华医院感染学杂志,2017,27(13):2928-2931. ZHANG Li, ZHANG Baochao, WANG Lu,et al.Prevalence of cytotoxin-associated gene a Helicobacter pylori infection in patients with coronary atherosclerotic heart disease[J].Chin J Nosocomiol,2017,27(13):2928-2931. [9] 韩宏毅,方文胜,张艳.细胞毒素相关基因A蛋白幽门螺杆菌感染对冠心病患者冠状动脉病变的影响[J].中华医院感染学杂志,2015,25(15):3397-3398,3401. HAN Hongyi,FANG Wensheng,ZHANG Yan.Influence of cytotoxin-associated gene a infected by Helicobacter pylori on the coronary artery lesions of patients with coronary heart disease[J].Chin J Nosocomiol,2015,25(15):3397-3398,3401. [10] 沈云峰,胡远贵,张洪波.细胞毒素相关蛋白毒力型幽门螺杆菌感染与冠心病患者血清炎性因子及同型半胱氨酸水平的相关性研究[J].中国循环杂志,2015,30(2):119-122. SHEN Yunfeng,HU Yuangui,ZHANG Hongbo.Relationship between Helicobacter pylori infection and inflammatory cytokines,homocysteine in patients with coronary artery disease[J].Chinese Circulation Journal,2015,30(2):119-122. [11] MENDALL M A,GOGGIN P M,MOLINEAUX N,et al.Relation of Helicobacterpylori infection and coronary heart disease[J].Br Heart J,1994,71(5):437-439. [12] JIANG J,CHEN Y,SHI J,et al.Population attributable burden of Helicobacter pylori-related gastric cancer,coronary heart disease,and ischemic stroke in China[J].Eur J Clin Microbiol Infect Dis,2017,36(2):199-212. [13] 张忠夫,戴玉柱,金美彤,等.幽门螺杆菌粪便抗原和血清抗体胶体金试验联合检测在体检人群的应用价值[J].现代检验医学杂志,2009,24(4):54-56. ZHANG Zhongfu,DAI Yuzhu,JIN Meitong,et al.Value of combinative application of stool HP antigen and serum HP antibody gold colloid tests in healthy examination crowd[J].J Mod Lab Med,2009,24(4):54-56. [14] 陈晶,刘明,晁艳茹,等.幽门螺杆菌感染对冠心病患者相关炎性因子的影响分析[J].中华医院感染学杂志,2016,26(15):3414-3416. CHEN Jing,LIU Ming,CHAO Yanru,et al.Effect of Helicobacter pyloriinfection on related inflammatory cytokines in patients with coronary heart disease[J].Chin J Nosocomiol,2016,26(15):3414-3416. [15] SUN Jing,RANGAN P,BHAT S S,et al.A meta-analysis of the associationbetween Helicobacter pylori infection and risk of coronary heart disease from published prospective studies[J].Helicobacter,2016,21(1):11-23.

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备注/Memo

备注/Memo:
作者简介:高建华(1982-),男,本科,主管技师,主要从事临床生化检验诊断研究,E-mail: gih.852@163.com。收稿日期:2019-06-13 修回日期:2019-06-28
更新日期/Last Update: 2019-07-30