[1]周 莹,管晓龙,李晓军,等.ELISA 检测KRT10_C 和COL6A3_C 短肽抗体的方法建立及作为RA 诊断的应用价值[J].现代检验医学杂志,2020,35(01):1-4.[doi:10.3969/j.issn.1671-7414.2020.01.001]
　ZHOU Ying,GUAN Xiao-long,LI Xiao-jun,et al.Establishment of ELISA for Detection of KRT10_C and COL6A3_C Short PeptideAntibodies and Its Application as A Diagnostic Biomarker for RA[J].Journal of Modern Laboratory Medicine,2020,35(01):1-4.[doi:10.3969/j.issn.1671-7414.2020.01.001]
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ELISA 检测KRT10_C 和COL6A3_C 短肽抗体的方法建立及作为RA 诊断的应用价值 ()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年01期
页码:
1-4
栏目:
论　　著
出版日期:
2020-02-29

文章信息/Info

Title:
Establishment of ELISA for Detection of KRT10_C and COL6A3_C Short PeptideAntibodies and Its Application as A Diagnostic Biomarker for RA
文章编号:
1671-7414(2020)01-001-05
作者:
周 莹1; 管晓龙2; 李晓军3; 虞 伟3; 管世鹤1
(1.安徽医科大学第二附属医院检验科,合肥 230601; 2.安徽医科大学附属妇幼保健院检验科,合肥 230001; 3.东部战区总医院中心实验科,南京 210002)
Author(s):
ZHOU Ying1;  GUAN Xiao-long2;  LI Xiao-jun3;  YU Wei3;  GUAN Shi-he1(1.
Department of Clinical Laboratory, the SecondAffiliated Hospital of Anhui Medical University, Hefei 230601, China; 2. Department of Clinical Laboratory, Maternal and Child Health Care Hospital,Affiliated to Anhui Medical University,Hefei 230001, China; 3.Department of Central Laboratory,Eastem Theater General Hospital,Nanjing 210002,China)
关键词:
类风湿关节炎;  角蛋白Ⅰ型细胞骨架10(KRT10)_C短肽抗体;  Ⅵ型胶原蛋白A3(COL6A3)_C短肽抗体;  酶联免疫吸附试验检测
分类号:
R446.61; R593.22
DOI:
10.3969/j.issn.1671-7414.2020.01.001
文献标志码:
A
摘要:
目的 建立角蛋白I型细胞骨架10[keratin type I cytoskeletal 10,KRT10)和VI型胶原蛋白A3[collagen alpha-3(VI)chain,COL6A3]短肽抗体的ELISA检测方法,并探讨两种瓜氨酸化短肽抗体在类风湿关节炎(rheumatoid arthritis, RA)实验室诊断中的价值。方法 以合成短肽为包被抗原,抗人IgA,IgG及IgM为二抗,检测100例抗瓜氨酸化蛋白抗体(anti-citrullinated protein antibodies,ACPA)阳性组、100例健康对照组和29例RA确诊患者血清中的KRT10,KRT10_C,COL6A3及COL6A3_C短肽抗体水平,比较不同短肽抗体与RA的相关性,采用ROC曲线分析KRT10_C和COL6A3_C短肽抗体对于RA的诊断价值,并对此ELISA方法进行精密度评价。结果 与临床诊断相比,KRT10_C短肽抗体诊断RA的灵敏度为58.62%,特异度为52.17%,用于诊断抗CCP抗体阳性RA患者的ROC曲线下面积达0.895; COL6A3_C短肽抗体诊断RA的灵敏度为65.52%,特异度为78.95%,用于诊断抗CCP抗体阴性的RA患者的ROC曲线下面积可达0.956。ELISA检测值KRT10_C(以抗人IgG为二抗)的批内变异系数分别为11.2%(低值)、7.8%(中值)和6.7%(高值)。ELISA检测COL6A3_C(以抗人IgM为二抗)的批内变异系数分别为12.9%(低值)、8.4%(中值)和8.9%(高值)。结论 KRT10_C和COL6A3_C短肽抗体对RA诊断具有重要意义,有望加入并完善实验室诊断体系,提高RA的早期诊断率。
Abstract:
Objective To establish an ELISA method for the detection of anti-KRT10_C antibody and anti-COL6A3_C antibody, and to explore the value of two citrullinated antibodies for the diagnosis of RA. Methods Synthetic short peptides were used as coating antigens, anti-human IgA, IgG and IgM were secondary antibodies. KRT10, KRT10_C, COL6A3 and COL6A3_C were detected in serum of 100 ACPA positive groups, 100 healthy controls and 29 RA patients. The peptide antibody levels were compared and the correlation between different short peptide antibodies and RA was compared. The diagnostic value of KRT10_C and COL6A3_C short peptide antibodies for RA was analyzed by ROC curve, and the precision of this ELISA method was evaluated. Results Compared with clinical diagnosis, the sensitivity of KRT10_C short peptide antibody for diagnosis of RA was 58.62%, the specificity was 52.17%, and the area under the ROC curve for the diagnosis of anti-CCP antibody(+)RA patients was 0.895. COL6A3_C short peptide antibody diagnosis RA The sensitivity was 65.52% and the specificity was 78.95%. The area under the ROC curve for RA patients diagnosed with anti-CCP antibody(-)was up to 0.956. The coeffici conclusion ents of variation of the ELISA test value KRT10_C(with anti-human IgG)were 11.2%(low), 7.8%(median)and 6.7%(high),respectively. The coefficients of variation of COL6A3_C(with anti-human IgM)were 12.9%(low),8.4%(median)and 8.9%(high),respectively.Conclusion KRT10_C and COL6A3_C short peptide antibodies are of great significance in the diagnosis of RA, and it is expected to add and improve the laboratory diagnosis system to improve the early diagnosis rate of RA.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81470071),国家临床重点专科建设项目(2014ZDZK003-1),江苏省临床医学科技专项(BL2014072),军区医药卫生科研重大专项(14ZX17)。 作者简介:周莹(1993-),女,硕士研究生,研究方向:临床检验诊断学,E-mail: zhouying161006@126.com。 通讯作者: 管世鹤,教授,主任技师,博士生导师,E-mail: shiheguan@126.com。 管晓龙,硕士,检验师,E-mail:guanxiaolong523@126.com。收稿日期:2019-10-20 修回日期:2019-11-19
更新日期/Last Update: 2020-03-30