[1]黄秋兰,钱巧慧,范德平,等.鱼腥草联合亚胺培南对碳青霉烯耐药铜绿假单胞菌杀菌效果的体外研究[J].现代检验医学杂志,2021,36(03):118-121.[doi:10.3969/j.issn.1671-7414.2021.03.027]
 HUANG Qiu-lana,QIAN Qiao-hui,FAN De-pinga,et al.Vitro Study on the Bactericidal Effect of Houttuynia Cordata Combined withImipenem Against Carbapenem Resistant Pseudomonas Aeruginosa[J].Journal of Modern Laboratory Medicine,2021,36(03):118-121.[doi:10.3969/j.issn.1671-7414.2021.03.027]
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鱼腥草联合亚胺培南对碳青霉烯耐药铜绿假单胞菌杀菌效果的体外研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年03期
页码:
118-121
栏目:
研究简报·实验技术
出版日期:
2021-05-31

文章信息/Info

Title:
Vitro Study on the Bactericidal Effect of Houttuynia Cordata Combined withImipenem Against Carbapenem Resistant Pseudomonas Aeruginosa
文章编号:
1671-7414(2021)03-118-04
作者:
黄秋兰钱巧慧范德平
(1. 上海市嘉定区南翔医院 a. 检验科 ;b. 中医科,上海 201802;2. 上海健康医学院附属周浦医院教学培训部,上海 201318)
Author(s):
HUANG Qiu-lan1aQIAN Qiao-hui2 FAN De-ping1aLIU Hong-chou1aXUE Na-li1aGAO Shou-xia1aMA Ying-wei1aZHOU Jia-ping1b
(1a.Department of Clinical Laboratory; 1b. Department of Traditional Chinese Medicine,Shanghai Jiading DsitrictNanxiang Hospital,Shanghai 201802,China; 2.Department of Teaching & Training, Shanghai Health and ScienceSchool Affiliated Zhoupu Hospital, Shanghai 201318, China)
关键词:
铜绿假单胞菌亚胺培南鱼腥草最低抑菌浓度
分类号:
R378.991;R446.5
DOI:
10.3969/j.issn.1671-7414.2021.03.027
文献标志码:
A
摘要:
目的 探究鱼腥草联合亚胺培南对碳青霉烯耐药铜绿假单胞菌(carbapenem resistant pseudomonas aeruginosa,CRPA)的体外杀菌效果,为合理使用抗生素、预防和控制医院感染提供参考。方法 样本取自上海市某医院收治患者送检的痰、尿、创面等液体标本中分离到的 50 株 CRPA,观察单药使用亚胺培南、鱼腥草及二者联合使用时对铜绿假单胞菌(PA)的最低抑菌浓度(MIC)、分级抑菌浓度(FIC)指数和对浮游菌的时间杀菌曲线。结果 亚胺培南单用的 MIC50(32 mg/L) 和 MIC90(128 mg/L) 均高于鱼腥草单用 (MIC502 mg/L, MIC904 mg/L) 及两者联合使用 (MIC500.25 mg/L,MIC901 mg/L),且鱼腥草单用的 MIC50 和 MIC90 均高于两者联合使用。分级抑菌浓度指数显示 12 株(24.0%)亚胺培南和鱼腥草联用后呈现协同作用,30 株(60.0%)表现相加作用,8 株(16.0%)表现无相关作用,未发现拮抗作用。随机选取 2 株菌株观察浮游菌的时间杀菌曲线,单独使用亚胺培南 6~12h 后,菌株均出现细菌恢复生长的现象;鱼腥草联合亚胺培南使用时,菌株的浮游菌数量降低均> 6log CFU/L,且 24h 时均无细菌生长现象。结论 鱼腥草联合亚胺培南优于单药亚胺培南的抗 CRPA 效率。

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备注/Memo

备注/Memo:
基金项目:上海市嘉定区科学技术委员会基金(JDKW-2018-W18)。
作者简介:黄秋兰(1976-),女,副主任技师,主要从事多重耐药菌研究,E-mail: huang15821947258@126.com。
通讯作者:周嘉平,E-mail: huhua021@126.com。
更新日期/Last Update: 1900-01-01