[1]杨晓玲,安 敏,李 玥,等.2 型糖尿病患者血清miR-103a 和miR-497 水平表达与微血管病变发生的相关性研究[J].现代检验医学杂志,2024,39(03):42-47.[doi:10.3969/j.issn.1671-7414.2024.03.007]
　YANG Xiaoling,AN Min,LI Yue,et al.Study on Correlation between Serum miR-103a, miR-497 Expression and Micro Vascular Disease in Patients with Type 2 Diabetes Mellitus[J].Journal of Modern Laboratory Medicine,2024,39(03):42-47.[doi:10.3969/j.issn.1671-7414.2024.03.007]
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2 型糖尿病患者血清miR-103a 和miR-497 水平表达与微血管病变发生的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年03期
页码:
42-47
栏目:
论著
出版日期:
2024-05-15

文章信息/Info

Title:
Study on Correlation between Serum miR-103a, miR-497 Expression and Micro Vascular Disease in Patients with Type 2 Diabetes Mellitus
文章编号:
1671-7414（2024）03-042-06
作者:
杨晓玲; 安 敏; 李 玥; 孙 玮; 马 亮
（唐山职业技术学院附属医院内分泌科，河北唐山063006）
Author(s):
YANG Xiaoling;  AN Min;  LI Yue;  SUN Wei;  MA Liang
(Department of Endocrinology, Affiliated Hospital of Tangshan Vocational & Technical College, Heibei Tangshan 063006, China)
关键词:
2 型糖尿病; 微血管病变; 微小核糖核酸-103a; 微小核糖核酸-497
分类号:
R587.2；R392.11
DOI:
10.3969/j.issn.1671-7414.2024.03.007
文献标志码:
A
摘要:
目的 探究2 型糖尿病（type 2 diabetes mellitus，T2DM）患者血清微小RNA-103a（miR-103a），微小RNA-497（miR-497）水平表达与微血管病变（micro vascular disease，MVD）发生的相关性。方法 选取2022 年5 月～ 2023年4 月在唐山市协和医院确诊的T2DM 患者作为研究组（n=113），根据是否并发MVD 分为2 型糖尿病微血管病变（type2 diabetes microangiopathy，DMAP）组（n=51）和T2DM（n=62）组，选取同期体检健康人员作为对照组（n=105）。收集患者临床资料，实时荧光聚合酶链反应（real-time fluorescence polymerase chain reaction，qRT-PCR）检测血清miR-103a 和miR-497 水平，采用Logistic 回归分析T2DM 并发MVD 的影响因素，绘制受试者工作特征（receiver operatingcharacteristic，ROC）曲线分析血清miR-103a 和miR-497 水平对T2DM 并发MVD 的诊断价值。结果 研究组血清miR-103a（0.62 ± 0.13）和miR-497（0.79 ± 0.14）水平显著低于对照组（0.96 ± 0.16，1.03 ± 0.18），差异具有统计学意义（t=17.273，11.031，均P<0.05）。DMAP 组血清miR-103a（0.53 ± 0.08）和miR-497（0.69 ± 0.10）水平显著低于T2DM 组（0.69 ± 0.10，0.87 ± 0.13），差异具有统计学意义（t=9.247，8.108，均P<0.05）。DMAP 组病程≥ 8 年、并发高血脂患者比例、空腹血糖（FBG）、总胆固醇（TC）、血肌酐（Scr）和尿素氮（BUN）水平显著高于T2DM组，差异具有统计学意义（t/χ2=8.294，15.342，-2.855，-5.659，-8.951，-3.880，均P<0.05）。血清miR-103a，miR-497 及二者联合诊断T2DM 并发MVD 的曲线下面积（area under curve，AUC）分别为0.899，0.897 和0.970，二者联合诊断效果优于各自单独诊断（Z=2.268，2.267，均P<0.05）。Logistic 多因素回归分析结果显示FBG（OR=1.879，95%CI：1.262~2.797），TC（OR=2.141，95%CI：1.348~3.400） 和Scr（OR=3.417，95%CI：1.569~7.440） 是T2DM并发MVD 的独立风险因素（均P<0.05），miR-103a（OR=0.784，95%CI：0.648 ～ 0.948）和miR-497（OR=0.839，95%CI：0.750 ～ 0.938）是T2DM 并发MVD 的保护因素（均P<0.05）。结论 T2DM 并发MVD 患者血清miR-103a和miR-497 低表达，其可能成为诊断T2DM 并发MVD 的潜在标志物。
Abstract:
Objective To explore the correlation between the expression of serum microRNA (miR) -103a and microRNA (miR) -497 and micro vascular disease (MVD) in patients with type 2 diabetes mellitus (T2DM). Methods Patients diagnosed with T2DM in Tangshan Union Medical College Hospital from May 2022 to April 2023 were selected as the study group (n=113), which were divided into type 2 diabetes microangiopathy (DMAP) group (n=51) and T2DM group (n=62) according to whether MVD was complicated. Healthy subjects in the same period were selected as control group (n=105). Clinical data were collected, and real-time fluorescence polymerase chain reaction (qRT-PCR) was applied to detect serum levels of miR-103a and miR-497. Logistic regression was applied to analyze the influencing factors of T2DM complicated with MVD. Receiver operating characteristic (ROC) curve was plotted to analyze the diagnostic value of serum miR-103a and miR-497 levels in T2DM complicated with MVD. Results The serum levels of miR-103a (0.62 ± 0.13) and miR-497 (0.79 ± 0.14) in the study group were lower than those in the control group (0.96 ± 0.16, 1.03 ± 0.18), with significant differences (t=17.273, 11.031, all P<0.05). The serum levels of miR-103a (0.53 ± 0.08) and miR-497 (0.69 ± 0.10) in the DMAP group were lower than those in the T2DM group (0.69 ± 0.10, 0.87 ± 0.13), with significant differences (t=9.247, 8.108, all P<0.05). The proportions of patients with a disease course of ≥ 8 years and concomitant hyperlipidemia, and the levels of FBG, TC, Scr and BUN in the DMAP group were higher than in the T2DM group, with significant differences (t/χ2=8.294, 15.342,-2.855,-5.659, -8.951, -3.880, all P<0.05). The area under curve (AUC) of serum miR-103a, miR-497 and their combination in the diagnosis of T2DM with MVD were 0.899, 0.897 and 0.970, respectively. The combined diagnosis effect was better than that of individual diagnosis (Z=2.268, 2.267, all P<0.05). The results of logistic regression analysis showed that FBG (OR=1.879, 95%CI: 1.262~2.797), TC (OR=2.141, 95%CI: 1.348~3.400), and Scr (OR=3.417, 95%CI: 1.569~7.440) were independent risk factors for T2DM with MVD (all P<0.05), while miR-103a (OR=0.784, 95%CI: 0.648 ～ 0.948) and miR-497 (OR=0.839, 95%CI: 0.750~0.938) were protective factors for T2DM with MVD (all P<0.05). Conclusion The serum miR-103a and miR-497 were low in patients with T2DM complicated with MVD, which may be potential markers for the diagnosis of T2DM complicated with MVD.

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备注/Memo

备注/Memo:
基金项目：2022 年度河北省医学科学研究课题计划（NO.20220219）：糖尿病患者肠道菌群与微血管功能的改变及药物治疗。
作者简介： 杨晓玲（1985-）女，本科，主治医师，研究方向：糖尿病、骨质疏松及其相关并发症诊治，甲状腺、肾上腺相关疾病的机理研究， E-mail：r47mlh@163.com。
通讯作者： 马亮（1980-）男，本科，主治医师，研究方向：糖尿病、骨质疏松及其相关并发症诊治、肾上腺相关疾病的机理研究，E-mail：d89qqg@163.com。
更新日期/Last Update: 2024-05-15