[1]李丽华a,史春艳b,张 欢a,等.老年2型糖尿病患者血清USP14和GRB2水平与下肢血管病变的相关性研究[J].现代检验医学杂志,2025,40(02):140-144.[doi:10.3969/j.issn.1671-7414.2025.02.026]
　LI Lihuaa,SHI Chunyanb,ZHANG Huana,et al.Study on the Correlation between Serum USP14 and GRB2 Levels and Vascular Lesions of the Lower Limbs in Elderly Patients with T2DM[J].Journal of Modern Laboratory Medicine,2025,40(02):140-144.[doi:10.3969/j.issn.1671-7414.2025.02.026]
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老年2型糖尿病患者血清USP14和GRB2水平与下肢血管病变的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年02期
页码:
140-144
栏目:
论著
出版日期:
2025-03-15

文章信息/Info

Title:
Study on the Correlation between Serum USP14 and GRB2 Levels and Vascular Lesions of the Lower Limbs in Elderly Patients with T2DM
文章编号:
1671-7414（2025）02-140-05
作者:
李丽华a; 史春艳b; 张 欢a; 柴雪妍a
（北京核工业医院 a. 内分泌科；b. 检验科，北京 102413）
Author(s):
LI Lihuaa; SHI Chunyanb; ZHANG Huana; CHAI Xueyana
（a. Department of Endocrinology；b. Department of Clinical Laboratory，Beijing Nuclear Industry Hospital，Beijing 102413，China）
关键词:
2 型糖尿病; 泛素特异性蛋白酶14; 生长因子受体结合蛋白2; 下肢血管病变
分类号:
R587.1；R392.11
DOI:
10.3969/j.issn.1671-7414.2025.02.026
文献标志码:
A
摘要:
目的 探讨老年2 型糖尿病（T2DM）患者血清泛素特异性蛋白酶14（USP14）和生长因子受体结合蛋白2（GRB2）水平与下肢血管病变（LEAD）的相关性。方法 选取北京核工业医院2020 年7 月～ 2023 年8 月收治的老年T2DM 患者153 例，根据有无LEAD 分为病变组（n=64）和非病变组（n=89）；另选取同期与老年T2DM 患者年龄相匹配的153 例健康体检志愿者作为对照组。采用酶联免疫吸附法（ELISA）检测患者血清USP14 和GRB2 水平。多因素Logistic 回归分析影响T2DM 患者LEAD 发生的因素；受试者工作特征曲线（ROC）分析老年T2DM 患者USP14，GRB2 水平对发生LEAD 的诊断价值。结果 与对照组相比，病变组和非病变组中血清USP14（8.56±1.57ng/ml，6.34±1.27ng/ml vs 4.23±1.02ng/ml），GRB2 水平（7.45±1.56ng/ml，5.23±1.12ng/ml vs 3.19±0.78ng/ml）明显增加，差异具有统计学意义（q=33.555，18.259；37.326,19.960，均P ＜ 0.05）。A 级、B 级和C 级血清USP14（7.14±1.31ng/ml，8.67±1.52ng/ml，9.98±2.01ng/ml），GRB2（6.31±1.25ng/ml，7.47±1.58ng/ml，8.69±1.74ng/ml）水平依次增加，差异具有统计学意义（F=14.754，11.404，均P ＜ 0.05）。多因素Logistic 回归分析结果显示，USP14，GRB2，TC，TG，LDL-C 和HbA1c 均是影响老年T2DM 患者LEAD 发生的影响因素（Wald χ2=11.618 ～ 41.458，均P ＜ 0.05）；ROC曲线结果显示，血清USP14 和GRB2 联合诊断LEAD的曲线下面积（AUC）高于USP14 和GRB2 单独诊断（Z=2.706，3.124，均P ＜ 0.05）。结论 USP14 和GRB2 在老年T2DM 并发LEAD 患者血清中均呈高表达水平，随LEAD 分级增加而升高，可作为诊断老年T2DM 并发LEAD 的血清标志物，两者联合检测效能更佳。
Abstract:
Objective To investigate the correlation between serum ubiquitin-specific protease 14 (USP14) and growth factor receptor binding protein 2 (GRB2) levels and vascular lesions of the lower limbs (LEAD) in elderly patients with type 2 diabetes (T2DM). Methods 153 elderly T2DM patients admitted to Beijing Nuclear Industrial Hospital from July 2020 to August 2023 were selected and divided into lesion group (n=64) and non-lesion group (n=89) according to the presence or absence of LEAD, in addition and 153 healthy volunteers who matched the age of elderly T2DM patients in the same period were selected as the control group. Enzyme-linked immunosorbent assay (ELISA) was used to detect serum USP14 and GRB2 levels in patients. Multifactorial Logistic regression was used to analyze the factors affecting the occurrence of LEAD in patients with elderly T2DM. Receiver operating curve (ROC) analysis of USP14 and GRB2 levels in elderly T2DM patients for the diagnostic value of LEAD occurrence. Results Compared with the control group, serum USP14（8.56±1.57ng/ml， 6.34±1.27ng/ml vs 4.23±1.02ng/ml）, GRB2 levels（7.45±1.56ng/ml，5.23±1.12ng/ml vs 3.19±0.78ng/ml）were significantly increased in the diseased and non-diseased groups, and the differences were statistically significant (q=33.555， 18.259；37.326，19.960，all P < 0.05). Class A, B and C serum USP14（7.14±1.31ng/ml，8.67±1.52ng/ml，9.98±2.01ng/ ml）, GRB2 levels（6.31±1.25ng/ml，7.47±1.58ng/ml，8.69±1.74ng/ml） increased in that order, and the differences were all statistically significant (F=14.754, 11.404, all P < 0.05). Multifactorial Logistic regression analysis showed that USP14 , GRB2, TC, TG，LDL-C and HbA1c were all influencing factors affecting the occurrence of LEAD in patients with T2DM (Wald χ2=11.618 ～ 41.458, all P< 0.05). The results of the ROC curve showed that the area under the curve (AUC) of the combined diagnosis of LEAD by serum USP14 and GRB2 was greater than that of USP14 and GRB2 diagnosed alone (Z=2.706, 3.124, all P<0.05). Conclusion USP14 and GRB2 were both highly expressed in the serum of patients with elderly T2DM combined with LEAD, which increased with the increase of LEAD grade, and can be used as serum markers for the diagnosis of elderly T2DM combined with LEAD, and the efficacy of the combined detection of the two is better.

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备注/Memo

备注/Memo:
作者简介：李丽华（1982-）女，大专，主治医师，研究方向：糖尿病，E-mail：t31hrw@163.com。
通讯作者：柴雪妍（1978-）女，本科，副主任医师，研究方向：糖尿病及并发症，E-mail：b81wny@163.com。
更新日期/Last Update: 2025-03-15