[1]陈 芬,许春华,姚 彤,等.儿童哮喘患者外周血 Th17,Th2 细胞及不同来源样本相关细胞因子表达与气道炎症诱导痰细胞学分类的相关性研究[J].现代检验医学杂志,2021,36(03):62-67.[doi:10.3969/j.issn.1671-7414.2021.03.014]
 CHEN Fen,XU Chun-hua,YAO Tong,et al.Study on the Correlation between the Expression of Th17, Th2 Cells andCytokines in Peripheral Blood and the Cytological Classification of AirwayInflammation-induced Sputum in Children with Asthma[J].Journal of Modern Laboratory Medicine,2021,36(03):62-67.[doi:10.3969/j.issn.1671-7414.2021.03.014]
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儿童哮喘患者外周血 Th17,Th2 细胞及不同来源样本相关细胞因子表达与气道炎症诱导痰细胞学分类的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年03期
页码:
62-67
栏目:
论 著
出版日期:
2021-05-31

文章信息/Info

Title:
Study on the Correlation between the Expression of Th17, Th2 Cells andCytokines in Peripheral Blood and the Cytological Classification of AirwayInflammation-induced Sputum in Children with Asthma
文章编号:
1671-7414(2021)03-062-06
作者:
陈 芬许春华姚 彤
(新疆维吾尔自治区儿童医院儿内科,乌鲁木齐 830001)
Author(s):
CHEN Fen XU Chun-hua YAO Tong AN Mei-yu LUO Xin-hui
(Department of Medicine, Children’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001,China)
关键词:
儿童哮喘辅助性 T 细胞 2辅助性 T 细胞 17气道炎症
分类号:
R725.6;R392.11
DOI:
10.3969/j.issn.1671-7414.2021.03.014
文献标志码:
A
摘要:
目的 探讨不同类型哮喘儿童外周血 Th2,Th17 细胞及不同来源样本相关细胞因子表达与气道炎症诱导痰细胞学分类的相关性。方法 选取 2018 年 4 月 ~2020 年 8 月新疆维吾尔自治区儿童医院接诊的急性发作期哮喘患儿 55 例行诱导痰中性粒细胞百分比(NEU%)和嗜酸性粒细胞百分比(EOS%)计数;根据其结果分为中性粒细胞哮喘组(NA 组,n=24)和嗜酸性粒细胞哮喘组(EA 组,n=31);另选取同期健康儿童 50 例作为对照组(HC 组)。采用流式细胞仪检测外周血 Th17,Th2 细胞及 Th17 细胞中 STAT5 和 BCL-2 表达;ELISA 法检测外周血单核细胞(PBMC)培养液和支气管肺泡灌洗液(BALF)中 IL-17 和 IL-10 表达;qRT-PCR 法检测外周血单核细胞 RORγt 表达;采用 Pearson 行相关性分析。 结果 NA 组 NEU% 高于 EA 组和 HC 组,EA 组高于 HC 组(F=342.918,P<0.001);EA 组 EOS% 高于 NA 组和HC 组,NA 组高于 HC 组(F=118.931,P<0.001),差异均有统计学意义。NA 组 Th17% 及 IL-17 水平高于 EA 组和 HC组,EA 组高于 HC 组(F=47.569~256.602,均 P<0.001);EA 组 Th2% 及 IL-10 水平高于 NA 组和 HC 组,NA 组高于HC 组(F=16.636~169.271,均 P<0.001),差异均有统计学意义。哮喘患儿诱导痰 NEU% 与 Th17%,IL-17 水平呈明显正相关(r=0.695~0.803,均 P<0.05);EOS% 与 Th2%,IL-10 水平呈明显正相关(r=0.684~0.759,均 P<0.05)。NA 组RORγt 表达及 BCL-2,STAT5 阳性率高于 EA 组和 HC 组,EA 组高于 HC 组,差异均有统计学意义(F=53.390~283.618,均 P<0.001)。结论 中性粒细胞哮喘患儿与嗜酸性粒细胞哮喘患儿气道炎症分别表现为诱导痰 NEU%,EOS% 明显增高,并分别与不同来源样本的 Th17%,IL-17 水平及 Th2% 和 IL-10 水平呈正相关,可将 Th2/ Th17 作为不同类型哮喘患儿诊断、治疗及预后的标志物。中性粒细胞性哮喘气道炎症与 Th17 细胞分化增强及已分化的 Th17 细胞存活延长密切相关。

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备注/Memo

备注/Memo:
基金项目:新疆维吾尔自治区卫生健康青年医学科技人才专项项目(编号:WJWY-202007)。
作者简介:陈芬(1984-),女,硕士研究生,主治医师,研究方向:儿童呼吸血液肿瘤专业,E-mail:howareyou3322@163.com。
通讯作者:罗新辉,女,硕士,主任医师,E-mail:5876275@qq.com.
更新日期/Last Update: 1900-01-01