[1]顾芯烨,吴旦平,江 波,等.ECE2通过上调E2F1和MYC来促进乳腺癌细胞的增殖和迁移[J].现代检验医学杂志,2021,36(04):39-44.[doi:10.3969/j.issn.1671-7414.2021.04.009]
 GU Xin-ye,WU Dan-ping,JIANG Bo,et al.ECE2 Promotes Proliferation and Migration of Breast Cancer Cells byUpregulation of E2F1 and MYC[J].Journal of Modern Laboratory Medicine,2021,36(04):39-44.[doi:10.3969/j.issn.1671-7414.2021.04.009]
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ECE2通过上调E2F1和MYC来促进乳腺癌细胞的增殖和迁移()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年04期
页码:
39-44
栏目:
论 著
出版日期:
2021-07-31

文章信息/Info

Title:
ECE2 Promotes Proliferation and Migration of Breast Cancer Cells byUpregulation of E2F1 and MYC
文章编号:
1671-7414(2021)04-039-06
作者:
顾芯烨1吴旦平1江 波1商江峰1蒋国勤2魏金荣2李 华3
(1. 常熟市第一人民医院甲乳外科,江苏常熟 215500;2. 苏州大学附属第二医院普外科,江苏苏州 215004;3. 府谷县人民医院,陕西榆林 719400)
Author(s):
GU Xin-ye1 WU Dan-ping1 JIANG Bo1 SHANG Jiang-feng1 JIANG Guo-qin2 WEI Jin-rong2 LI hua3
(1.Department of Breast and Thyroid Surgery,the First People’s Hospital of Changshu City,Jiangsu Changshu215500,China;2. Department of General Surgery,the Second Affiliated Hospital of Soochow University,JiangsuSuzhou 215004,China;3.Fugu County People’s Hospital, Shaanxi Yulin 719400, China)
关键词:
乳腺癌内皮素转化酶2E2F1MYC增殖迁移
分类号:
R737.9;R730.43
DOI:
10.3969/j.issn.1671-7414.2021.04.009
文献标志码:
A
摘要:
目的 通过检测内皮素转化酶2(ECE2)在乳腺癌中的表达,探究其对乳腺癌细胞增殖、迁移的影响及潜在分子机制。方法 选取20 例临床乳腺癌组织及其对应癌旁正常组织样本,通过qRT-PCR 检测ECE2 基因在乳腺癌组织中的表达特征。通过siRNA 介导敲低ECE2 表达,利用细胞增殖实验、克隆形成实验、划痕迁移实验和细胞周期实验分别验证敲低ECE2 表达对乳腺癌细胞增殖、克隆形成、迁移及凋亡的影响。通过分析与ECE2 共表达基因参与的信号通路,探索ECE2 在乳腺癌发展进程中的潜在分子机制,进一步通过细胞增殖回补实验进行验证。结果 20 例临床乳腺癌组织中ECE2 表达较癌旁正常组织显著上调(30.342±6.564 vs 21.753±8.244),差异有统计学意义(t=3.645,P=0.000)。转染敲低ECE2 表达后,siECE2#1 组和siECE2#2 组在24,48,72 h 的吸光度值较对照组明显降低,差异有统计学意义(F=71.409~211.681,均P < 0.01)。siECE2#1 组(0.515±0.014)和siECE2#2 组(0.511±0.006)细胞克隆形成速率明显低于对照组(3.473±0.147),差异有统计学意义(F=467.152,P < 0.001)。siECE2#1 组(56.423±1.137)和siECE2#2 组(42.036±0.754)细胞迁移愈合速率显著低于对照组(78.124±1.352),差异有统计学意义(F=805.162,P < 0.001)。siECE2#1 组和siECE2#2 组细胞凋亡率较对照组明显降低,细胞周期显著阻滞在G1 期。siECE2#1 组和siECE2# 组细胞中E2F1 mRNA 和蛋白表达水平明显低于对照组,差异均有统计学意义(F=703.020,136.301,均P< 0.001);MYC mRNA 和蛋白表达水平也明显低于对照组(F=613.395,102.573,均P < 0.001)。在敲低ECE2 表达的乳腺癌细胞中分别回补E2F1 和MYC 后,细胞增殖速率回归至正常水平。结论 ECE2 高表达诱导促癌基因E2F1和MYC 的高表达进而促进乳腺癌细胞的增殖及迁移,阻碍细胞周期停滞在G1 期,参与乳腺癌的发展进程。

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备注/Memo

备注/Memo:
基金项目:江苏省卫生计生委科研课题(Z201951)。
作者简介:顾芯烨(1987-),男,硕士研究生,主治医师,研究方向:乳腺癌基础与临床研究,E-mail:g17416561@163.com。
通讯作者:李华(1973-),女,本科,副主任技师,研究方向:临床检验,E-mail:512954051@qq.com。
更新日期/Last Update: 1900-01-01