[1]邓刚,张平安.慢性丙型肝炎男性患者体内雌雄激素与抗黏病毒基因表达的关系[J].现代检验医学杂志,2015,30(05):53-55.[doi:10.3969/j.issn.1671-7414.2015.05.016]
　DENG Gang,ZHANG Ping-an.Role of Estrogen and Androgen on Gene Expression of Myxovirus Resistance A in Male Patients with Chronic Hepatitis C[J].Journal of Modern Laboratory Medicine,2015,30(05):53-55.[doi:10.3969/j.issn.1671-7414.2015.05.016]
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慢性丙型肝炎男性患者体内雌雄激素与抗黏病毒基因表达的关系()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年05期
页码:
53-55
栏目:
论著
出版日期:
2015-12-10

文章信息/Info

Title:
Role of Estrogen and Androgen on Gene Expression of Myxovirus Resistance A in Male Patients with Chronic Hepatitis C
作者:
邓刚; 张平安
武汉大学人民医院检验科，武汉430060
Author(s):
DENG Gang; ZHANG Ping-an
Department of Clinical Laboratory，Renmin Hospital of Wuhan University，Wuhan 430060，China
关键词:
慢性丙型肝炎; 抗黏病毒基因A; 雌雄激素; 肝功能; 实时荧光定量PCR
分类号:
R512.63；Q786
DOI:
10.3969/j.issn.1671-7414.2015.05.016
文献标志码:
A
摘要:
目的探讨血清雌雄激素与慢性丙型肝炎(CHC)患者外周血单个核细胞(PBMC)中抗黏病毒基因A(MxA)mRNA表达和肝功能的关系。方法选择慢性丙型肝炎男性患者51例，健康人群49例。采用酶法检测肝功能指标；采用化学发光检测雌雄激素；用实时荧光定量PCR检测MxA基因mRNA表达。结果CHC患者血清睾酮、雌二醇、ALT，AST和MxA基因mRNA表达量与健康人群比较，差异均有统计学意义(t=-4.69，11.34，7.79，7.87，4.28；P＜0.05)。对CHC患者MxA基因表达量与血清中雌雄激素、肝功能进行相关性分析，结果发现MxA基因表达量仅与雌二醇呈反比(r=-0.479，P＜0.05)。结论HCV患者雌雄激素发生紊乱并且雌激素与MxA基因表达呈负相关。
Abstract:
ObjectiveTo explore the role of Estrogen and Androgen on gene expression of myxovirus resistance A of peripheral blood mononuclear cells (PBMC)，liver function in patients with chronic hepatitis C (CHC) infection．MethodsPeripheral blood was collected from 51 male patients with chronic hepatitis C infection，49 healthy individuals．Liver function was detected using enzymatic．Estrogen and Androgen were detected by Chemiluminescence．The expression of MxA mRNA was detected by real-time quantitative PCR．ResultsSerum estrogen，androgen，ALT，AST and expression of MxA mRNA were significant difference between CHC patients and healthy people (t=-4.69，11.34 7.79，7.87，4.28；P＜0.05)．Correlation analysis of MxA gene expression with serum estrogen and androgen，liver function in CHC patients was found that the expression of MxA mRNA was only negative correlation with estrogen (r=-0.479；P＜0.05)．ConclusionSerum estrogen and androgen were disorder and MxA gene expression was negative correlation with estrogen in CHC patients．

参考文献/References:

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备注/Memo

备注/Memo:
作者简介：邓刚(1981-)，男，在读研究生，研究方向：感染性疾病的免疫遗传学检验，Tel：15072218855，E-mail：93541637@qq.com。 通讯作者：张平安，E-mail：zhangpingan@aliyun.com。
更新日期/Last Update: 1900-01-01