[1]唐玉蓉a,王际亮a,孙婷婷a,等.儿童特发性血小板减少性紫癜Th亚群细胞因子的测定及意义[J].现代检验医学杂志,2015,30(05):143-145.[doi:10.3969/j.issn.1671-7414.2015.05.045]
　TANG Yu-ronga,WANG Ji-lianga,SUN Ting-tinga,et al.Determination and Clinical Significance of Th Subsets Cytokines in ITP of Children[J].Journal of Modern Laboratory Medicine,2015,30(05):143-145.[doi:10.3969/j.issn.1671-7414.2015.05.045]
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儿童特发性血小板减少性紫癜Th亚群细胞因子的测定及意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年05期
页码:
143-145
栏目:
检验与临床
出版日期:
2015-12-10

文章信息/Info

Title:
Determination and Clinical Significance of Th Subsets Cytokines in ITP of Children
作者:
唐玉蓉a; 王际亮a; 孙婷婷a; 伊心浩b
山东省胜利油田中心医院 a.检验科；b.科教科，山东东营257034
Author(s):
TANG Yu-ronga; WANG Ji-lianga; SUN Ting-tinga; YI Xin-haob
a.Department of Clinical Laboratory；b.Department of Science and Education， Central Hospital of Shengli Oil Field，Shandong Dongying 257034，China
关键词:
儿童; 特发性血小板减少性紫癜; 干扰素-γ; 白细胞介素-4; 转化生长因子-β1; 流式细胞术; 外周血单个核细胞
分类号:
R554.6；R392.12
DOI:
10.3969/j.issn.1671-7414.2015.05.045
文献标志码:
A
摘要:
目的研究外周血Th亚群细胞因子IFN-γ，IL-4和TGF-β1在急、慢性儿童特发性血小板减少性紫癜中的浓度及临床意义。方法应用流式细胞术检测外周血IFN-γ和IL-4表达水平，采用酶联免疫(ELISE)法检测外周血单个核细胞生成的TGF-β1水平，并与24例正常对照组检测进行比较。结果aITP患儿Th1细胞因子IFN-γ阳性百分比高于正常对照组(P<0.05)，cITP患儿Th1细胞因子IFN-γ阳性百分比较对照组显著降低(P<0.01)。aITP患儿Th2细胞因子IL-4与正常对照组比较差异无统计学意义(P>0.05)，cITP患儿Th2细胞比对照组显著升高(P<0.05)。aITP和cITP Th3细胞因子TGF-β1水平均较正常对照组明显降低，cITP较aITP显著降低，差异有统计学意义(P<0.05)。结论儿童急、慢性ITP中Th细胞因子对其发病机制研究及鉴别诊断有重要价值。
Abstract:
ObjectiveTo study the determination and clinical significance of Th cytokines subsets of IFN-γ，IL-4 factor and TGF-β1 in acute and chronic idiopathic thrombocytopenic purpura．MethodsThe level of IFN-γ，IL-4 and TGF-β1 (produced by peripheral blood mononuclear cells)were detected by flow cytometry and enzyme-linked immunosorbent assay (ELISE)，respectively，in patients and 24 controls．ResultsThe positive percentage of factor IFN-γ in children with aITP was higher than that in the controls (P<0.05)．The positive percentage of factor IFN-γ in children with cITP was significantly lower than the controls (P<0.01)．The level of IL-4 had no statistical significance in children with aITP compared with controls．The level of IL-4 was higher significantly in children with cITP than that in controls (P<0.05)．The level of TGF-β1 in children with aITP and cITP was lower significantly than that in controls，that was more significance in cITP than that in aITP (P<0.05)．ConclusionThe state of Th cytokines in children with acute and chronic ITP is of great value in the understanding of the pathogenesis and differential diagnosis．

参考文献/References:

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备注/Memo

备注/Memo:
作者简介：唐玉蓉(1975-)，女，学士，副主任技师，从事临床检验领域的研究及检验工作，Tel：0546-8770171，E-mail：zxyyjyktyr@163.com。
更新日期/Last Update: 1900-01-01