[1]董 莉,张保平,冯新平,等.血浆HSP90α检测在肺癌诊断中的临床应用价值研究[J].现代检验医学杂志,2017,32(04):97-100.[doi:10.3969/j.issn.1671-7414.2017.04.027]
　DONG Li,ZHANG Bao-ping,FENG Xin-ping,et al.Plasma HSP90 Alpha Testing Clinical Application Value in the Diagnosis of Lung Cancer Researc[J].Journal of Modern Laboratory Medicine,2017,32(04):97-100.[doi:10.3969/j.issn.1671-7414.2017.04.027]
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血浆HSP90α检测在肺癌诊断中的临床应用价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年04期
页码:
97-100
栏目:
论著
出版日期:
2017-07-25

文章信息/Info

Title:
Plasma HSP90 Alpha Testing Clinical Application Value in the Diagnosis of Lung Cancer Researc
文章编号:
1671-7414(2017)04-097-04
作者:
董 莉; 张保平; 冯新平; 王悦喜
内蒙古医科大学附属医院检验科,呼和浩特 010050
Author(s):
DONG Li; ZHANG Bao-ping; FENG Xin-ping; WANG Yue-xi
Department of Clinical Laboratory, Inner Mongolia Medical University Affiliated Hospital,Hohehot 010050,China
关键词:
热休克蛋白90α; 肺癌; 联合检测
分类号:
R734.2; R730.43
DOI:
10.3969/j.issn.1671-7414.2017.04.027
文献标志码:
A
摘要:
目的 探讨血浆热休克蛋白90α(HSP90α)检测在肺癌诊断中的意义。方法 应用酶联免疫法检测166例肺癌患者血浆HSP90α浓度,化学发光法检测CEA,NSE,SCC,CYFRA21-1; 同期健康体检者20例为正常对照组,两组数据采用t检验进行统计学分析; 采用Pearman's相关法分析肺癌患者HSP90α与CEA,NSE,SCC和CYFRA21-1间的关系; 采用ROC曲线评估HSP90α对肺癌的诊断效能。结果 ①肺癌组与对照组HSP90α,CEA,NSE,SCC,CYFRA21-1分别为190.33±105.86 vs 41.02±19.73 ng/ml,8.68±5.02 vs 4.02±1.36 ng/ml,36.32±13.16 vs 8.32±3.96 ng/ml,6.21±1.62 vs 1.23±0.64 ng/ml,10.63±4.33vs 3.02±1.66 ng/ml; 与对照组比较,HSP90α,CEA,NSE,SCC和CYFRA21-1明显高于健康对照组,差异有统计学意义(t=10.48,8.66,12.36,9.52,15.36,均P值<0.001)。②以生物参考区间(HSP90α:0～86 ng/ml,CEA 1.0～5 ng/ml,NSE:1.0～17.5 ng/ml,SCC:0.2～1.6 ng/ml,CYFRA21-1:1.0～2.6 ng/ml)为标准,肺癌组中,HSP90α增高占73.49%,CEA增高占19.27%,NSE增高占37.95%,CYFRA21-1增高占21.68%,SCC增高占29.51%。③肺癌患者按病理组织分型,不同肿瘤分型HSP90α浓度比较无差异(P>0.05)。④Spearman秩相关分析显示,HSP90α与CYFRA21-1水平呈正相关(rs=0. 44,P<0.01),差异有统计学意义(F=14.98,P=0.00); HSP90α与CEA,NSE,SCC无相关性。⑤HSP90α与CEA,NSE,SCC和CYFRA21-1诊断肺癌的ROC曲线下面积(AUC)分别为:0.961,0.562,0.731,0.465和0.632,最佳cutoff值分别为89.3 ng/ml,6.32 ng/ml,18.63 ng/ml,1.93 ng/ml和2.36 ng/ml; 灵敏度分别为73.49%,52.3%,63.2%,59.6%和62.1%:特异度分别为98.6%,46.3%,66.3%,61.3%和63.4%; 准确度分别为88.4%,80.3%,86.9%,87.2%和89.2%。五项联合诊断肺癌的灵敏度和特异度分别为100%和75%。结论 用ROC曲线分析的结果表明,HSP90α对诊断肺癌具有辅助作用,联合CEA,NSE,CYFRA21-1及SCC可显著提高肺癌的检出率。
Abstract:
Objective To study the plasma heat shock protein 90 alpha(HSP90 α)in the diagnosis of lung cancer.Methods Chose 166 cases of patients with lung cancer,lung cancer group,the samephysical examination of 20 cases of normal(control group),application of plasma concentration of HSP90 α enzyme-linked immunoassay detection,chemiluminescence detection of CEA,NSE,SCC and CYFRA21-1,of the two groups of data by t test statistical analysis,compared two groups of plasma HSP90 α level.Withplasma HSP90 alpha was greater than 86 ng/ml for the critical value,calculation of HSP90 α testing sensitivity.Patients with lung cancer by histopathologicclassification,compare different tumor classification in patients with plasma HSP90 α level.Used Pearman's correlation method to analyse the relationship ofHSP90 α,CEA and NSE in patients with lung cancer,between SCC and CYFRA21-1 andused ROC curve to evaluate HSP90 α efficiency to the diagnosis of lung cancer.Results ① In lung cancer group and control group in the indicators HSP90 α,CEA,NSE,SCC and respectively CYFRA21-1 190.33±105.86 vs 41.02±19.73 ng/ml,8.68±5.02 vs 4.02±1.36 ng/ml,36.32±13.16 vs8.32±3.96 ng/ml,6.21±1.62 vs 1.23±0.64 ng/ml,10.63±4.33 vs 3.02±1.66 ng/ml.Compared with control group(t=10.48,8.66,12.36,9.52,15.36,P<0.01),the difference was statistically significant.② For biological reference range(HSP90 α:0～86 ng/ml,CEA 1.0～5 ng/ml,NSE:1.0～17.5 ng/ml,SCC:0.2～1.6 ng/ml,CYFRA21-1:1.0～2.6 ng/ml)as the standard in lung cancer group,HSP90 α increased 73.49%,CEA increased 19.27%,NSE increased 19.27%,CYFRA21-1(21.68%)and SCC increased 29.51%.③ Patients with lung cancer by histopathologic classification,different concentration of tumor classification HSP90 α was no difference(P>0.05).④Spearman rank correlation analysis showed that HSP90 α levels were positively correlated with CYFRA21-1(rs=0.44,P<0.01).The difference was statistically significant(F=14.98,P=0. 00).HSP90 α and CEA,NSE,SCC had no relevance.⑤HSP90 α and CEA,NSE,SCC,CYFRA21-1 the area under the ROC curve(AUC)in the diagnosis of lungcancer were:0.961,0.562,0.731,0.465 and 0.632 best cutoff value were 89.3 ng/ml,6.32 ng/ml,18.63 ng/ml,1.93 ng/ml and 2.36 ng/ml.Sensitivityof 73.49%,52.3%,73.49%,59.6% and 62.1%,specific degrees respectively.Accuracy of 98.6%,46.3%,66.3%,98.6% and 46.3%,respectively,88.4%,80.3%,86.9%,87.2% and 89.2% of the five joint,the sensitivity of diagnosis oflung cancer and specific degrees respectively 100% and 75%.Conclusion Using ROC curve analysis showed that HSP90 α plays an auxiliaryrole in diagnosis of lung cancer,CEA,NSE,CYFRA21-1 and SCC can significantlyincrease the detection rate of lung cancer.

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备注/Memo

备注/Memo:
作者简介:董 莉(1964-),女,大学本科,硕士研究生导师,主任检验师,主要从事临床免疫检验及相关疾病研究,E-mail:dongsuanzhi@163.com。 通讯作者:王悦喜(1963-),医学博士。
更新日期/Last Update: 1900-01-01