[1]刘先宁,汪　耀,潘士印,等.人角膜基质间充质干细胞外泌体的分离制备及鉴定[J].现代检验医学杂志,2021,36(05):114-116.[doi:10.3969/j.issn.1671-7414.2021.05.025]
　LIU Xian-ning,WANG Yao,PAN Shi-yin,et al.Isolation and Identification of Exosomes Derived from Human CornealStromal Mesenchymal Stem Cells[J].Journal of Modern Laboratory Medicine,2021,36(05):114-116.[doi:10.3969/j.issn.1671-7414.2021.05.025]
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人角膜基质间充质干细胞外泌体的分离制备及鉴定()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年05期
页码:
114-116
栏目:
研究简报·实验技术
出版日期:
2021-10-14

文章信息/Info

Title:
Isolation and Identification of Exosomes Derived from Human CornealStromal Mesenchymal Stem Cells
文章编号:
1671-7414（2021）05-114-03
作者:
刘先宁; 汪　耀; 潘士印; 肖湘华; 银　勇; 王亚妮; 刘　睿; 朱秀萍; 赵　明
（西安市第一医院，陕西省眼科研究所，陕西省眼科学重点实验室，陕西省眼科疾病临床医学研究中心，西北大学附属第一医院，西安 710002）
Author(s):
LIU Xian-ning;  WANG Yao;  PAN Shi-yin;  XIAO Xiang-hua;  YIN Yong; WANG Ya-ni;  LIU Rui;  ZHU Xiu-ping;  ZHAO Ming
（Xi’an First Hospital, Shaanxi Institute of Ophthalmology,Shaanxi Key Lab of Ophthalmology, Clinical Research Center forOphthalmology Diseases of Shaanxi Province,the First Affiliated Hospital of Northwest University,Xi’an 710002, China）
关键词:
人角膜基质间充质干细胞; 外泌体; 制备及鉴定
分类号:
R772.2；R446
DOI:
10.3969/j.issn.1671-7414.2021.05.025
文献标志码:
A
摘要:
目的　制备人角膜基质间充质干细胞（human corneal stromal mesenchymal stem cells，HC-MSCs)外泌体并进行生物学鉴定。方法　采用组织块培养技术获得HC-MSCs，利用超速离心法从HC-MSCs培养上清中分离制备外泌体，通过蛋白免疫印迹、透射电镜、纳米颗粒跟踪分析法进行鉴定。结果　分离的纳米囊泡表达外泌体特异性标志蛋白CD63，CD81和TSG101,在透射电子显微镜下可见其呈圆形、卵圆形和杯盘状囊泡，直经113.3 nm。结论　从HC-MSCs培养上清中可分离出具有典型结构及特征的外泌体，为HC-MSCs外泌体的作用机制及其在眼科的转化应用研究奠定坚实的基础。
Abstract:
Objective　To isolation and identification of exosomes derived from human corneal stromal mesenchymal stem cells(HCS-MSCs) and analyze their biological characters. Methods　HCS-MSCs were obtained by tissue mass culture technique.Exosomes were isolated from the supernatant of HCS-MSCs culture through ultracentrifugation and the characteristic of theexosome were identified by Western blot, transmission electron microscopy and nanoparticle tracking analysis. Results　HCSMSCsderived exosomes expressed positive makers of CD63，CD81 and TGS101. It could be seen a classical doughnut shape ofexosomes through transmission electron microscope. The particles size displayed 113.3 nm. Conclusion　Exosomes with typicalstructures and characteristics could be isolated from supernatant culture of HCS-MSCs, which laid a foundation for furtherapplication of exosomes from HCS-MSCs in ophthalmology, which lays a foundation for further research on the mechanism ofaction and transformation and application of exosomes from HCS-MSCs in ophthalmology in ophthalmology.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：陕西省重点研发计划项目（2018ZDXM-SF-056 和2019SF-196） ；陕西省自然科学基础研究计划一般项目（面上）（No.2021JM-593）。
作者简介：刘先宁（1965-），女，本科，研究员，西安市有突出贡献青年专家，主要从事眼病基础及应用研究工作，E-mail:Lxn65 ＠ sina.com。
通讯作者：赵明（1981-），女，本科，主管检验师，专业：医学检验，E-mail:1603106922@ qq.com。
更新日期/Last Update: 1900-01-01