[1]王 路,叶 莎,韩 霞,等.循环外泌体miRNA 检测对肝细胞癌临床诊断效能的Meta 分析[J].现代检验医学杂志,2022,37(04):59-63.[doi:10.3969/j.issn.1671-7414.2021.04.012]
　WANG Lu,YE Sha,HAN Xia,et al.Meta-Analysis of the Diagnostic Value of Circulating Exosomes miRNA Detection for Hepatocellular Carcinoma[J].Journal of Modern Laboratory Medicine,2022,37(04):59-63.[doi:10.3969/j.issn.1671-7414.2021.04.012]
点击复制

循环外泌体miRNA 检测对肝细胞癌临床诊断效能的Meta 分析()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年04期
页码:
59-63
栏目:
论著
出版日期:
2022-07-15

文章信息/Info

Title:
Meta-Analysis of the Diagnostic Value of Circulating Exosomes miRNA Detection for Hepatocellular Carcinoma
文章编号:
1671-7414（2022）04-059-05
作者:
王 路1;  叶 莎1;  韩 霞1;  伏建峰2
1. 巴音郭楞蒙古自治州人民医院医学检验科, 新疆库尔勒 841000;2. 新疆军区总医院临床检验中心, 乌鲁木齐 830000
Author(s):
WANG Lu1;  YE Sha1; HAN Xia1; FU Jian-feng2
1. Department of Clinical Laboratory, the People’s Hospital of Bayingoleng Mongolian Autonomouis Prefecture, Xingjiang Kuehler 841000, China；2. Clinical Laboratory Diagnostics Center, Xinjiang Army General Hospital,Urumqi 830000, China
关键词:
微小核糖核酸; 外泌体; 肝细胞癌; meta 分析
分类号:
R735.7；R730.43
DOI:
10.3969/j.issn.1671-7414.2021.04.012
文献标志码:
A
摘要:
目的 Meta 分析评价循环外泌体微小核糖核酸（micro RNA, miRNA）对肝细胞癌（hepatocellular carcinoma，HCC）的临床诊断效能。方法 依托PubMed, Embase,Cochrane Library,Web of science, 中国期刊全文数据库（CNKI）、万方数据库、中国生物医学文献检索数据库（CBM）和维普咨讯中文期刊服务平台（VIP）检索并筛选从建库至2021年1 月21 日收录的循环外泌体miRNA 应用于HCC 临床诊断的相关文献，采用QUADAS-2（quality assessment ofdiagnostic accuracy studies）对纳入文献质量进行评价，应用Meta-Disc1.4 软件进行分析，计算合并敏感度、合并特异度、合并阳/ 阴性似然比和合并诊断比值比（diagnostic odds ratio, DOR）以及汇总受试者工作曲线（SROC）下面积，以评估诊断的准确性，进而绘制 Fagan’s 列线图进行临床效果评价。结果 按纳入、排除标准筛选获得10 篇文献，共计12 项研究，包括775 例HCC 患者和957 例非HCC 人群；依据各研究间存在由非阈值效应引起的异质性，采用随机效应模型合并效应量，循环外泌体miRNA 诊断HCC 的合并灵敏度、特异度、阳性似然比和阴性似然比分别为0.892，0.884，7.019 和0.122，以及合并DOR=63.695 和SROC 曲线下面积（AUC）为0.950 7（Q=0.891 4）。结论 循环外泌体miRNA 可作为一种潜在的新的生物标志物应用于HCC 临床诊断。
Abstract:
Objective To evaluate the clinical diagnostic efficacy of circulating exosomal miRNA in hepatocellular carcinoma (HCC). Methods Based on PubMed, Embase, Cochrane Libarary, Web of science, CNKI, WanFang, CBM and VIP database platforms, the relevant literatures on the application of circulating exosomal miRNA in the clinical diagnosis of HCC from the establishment of the database to January 21, 2021 were searched and screened. The quality of the included literatures was evaluated by QUADAS-2, and analyzed by Meta-Disc1.4 software to calculate the combined sensitivity，combined specificity, combined positive / negative likelihood ratio and combined diagnostic odds ratio (DOR)，and summarize the area under the subject working curve (SROC) to evaluate the accuracy of diagnosis, and then draw Fagan’s nomogram for clinical effect evaluation. Results According to the inclusion and exclusion criteria, 10 literatures were obtained, including 12 studies, including 775 HCC patients and 957 non HCC people. According to the heterogeneity caused by non threshold effect among studies, the effect quantity was combined by random effect model. The combined sensitivity, specificity, positive likelihood ratio, positive likelihood ratio were 0.892, 0.884, 7.019 and 0.122, respectively. The combined DOR = 63.695 and the area under SROC curve was 0.950 7, Q = 0.891 4. Conclusion Circulating secrete miRNA can be used as a potential new biomarker in the clinical diagnosis of HCC.

参考文献/References:

[1] FENG Ruimei, ZONG Yinan, CAO Sumei, et al.Current cancer situation in China: good or bad news from the 2018 Global Cancer Statistics[J]. Cancer Communications (London, England), 2019, 39(1): 22.
[2] VILLANUEVA A. Hepatocellular carcinoma[J].New England Journal of Medicine, 2019,380(15):1450-1462.
[3] SONG Peipei, CAI Yulong, TANG Haowen, et al.The clinical management of hepatocellular carcinoma worldwide: A concise review and comparison of current guidelines from 2001 to 2017[J]. Bioscience Trends,2017, 11(4): 389-398.
[4] CARRETERO-GONZ?LEZ A, OTERO I, CARRILAJURIA L, et al. Exosomes: definition, role in tumor development and clinical implications[J]. Cancer Microenvironment , 2018, 11(1): 13-21.
[5] 王路, 叶莎, 史清海. 外泌体在HBV 感染相关性肝脏疾病中的研究进展[J]. 国际检验医学杂志, 2020,41(14):1754-1758. WANG Lu, YE Sha, SHI Qinghai, et al. Research progress of exosomes in HBV infection-related liver diseases [J]. International Journal of Laboratory Medicine, 2020,41 (14): 1754-1758.
[6] 高斌, 熊莹晖, 黄泽炳, 等．乙肝相关性肝细胞癌患者血清外泌体miR-1290 水平的变化及其诊断价值[J]. 中国普通外科杂志, 2019,28 (1): 31-38. GAO Bin, XIONG Yinghui, HUANG Zebing, et al.Change in serum level of exosomal miR-1290 in patients with hepatitis B virus related hepatocellular carcinoma and its diagnostic value [J]. Chinese Journal of General Surgery, 2019,28 (1): 31-38.
[7] 张云, 庹敏, 刘胜武. 血清外泌体来源的miRNA-122对肝癌诊断的研究[J]. 检验医学与临床, 2017,14 (9):1260-1261, 1265. ZHANG Yun, TUO Min, LIU Shengwu. Vsalue of serum exosomes miRNA-122 detection in diagnosis of primary hepatocellular carcinoma [J]. Laboratory Medicine and Clinic, 2017,14 (9): 1260-1261, 1265.
[8] ZHANG Yigan, XI Huaze, NIE Xin, et al. Assessment of miR-212 and other biomarkers in the diagnosis and treatment of HBV-infection-related liver diseases[J].Current Drug Metabolism, 2019, 20(10): 785-798.
[9] WANG Yurong, ZHANG Chunyan, ZHANG Pengjun, et al.Serum exosomal microRNAs combined with alpha-fetoprotein as diagnostic markers of hepatocellular carcinoma[J]. Cancer Medicine, 2018,7(5): 1670-1679.
[10] CHO H J, EUN J W, BAEK G O, et al. Serum exosomal microRNA, miR-10b-5p, as a potential diagnostic biomarker for early-stage hepatocellular carcinoma[J].Journal of Clinical Medicine, 2020, 9(1): 281.
[11] ABD E A, MATBOLI M, EL-TAWDI A, et al. Role of exosomal competing endogenous RNA in patients with hepatocellular carcinoma[J]. Journal of Cellular Biochemistry, 2018, 119(10): 8600-8610.
[12] MATBOLI M, LABIB M E, NASSER H E, et al.Exosomal miR-1298 and lncRNA-RP11-583F2.2 expression in hepato-cellular carcinoma[J].Current Genomics, 2020, 21(1): 46-55.
[13] WANG Shuo, YANG Yongxiang, SUN Lili, et al.Exosomal microRNAs as liquid biopsy biomarkers in hepatocellular carcinoma[J]. Onco Targets and Therapy,2020, 13: 2021-2030.
[14] SHENG Langqing, LI Jiarong, QIN Hao, et al. Blood exosomal micro ribonucleic acid profiling reveals the complexity of hepatocellular carcinoma and identifies potential biomarkers for differential diagnosis[J]. World Journal of Gastrointestinal Oncology, 2020, 12(10):1195-1208.
[15] 汪晓华, 陈晓军. 肝癌患者外周血外泌体miR-25 和miR-141 表达水平及联合检测诊断效能研究[J]. 中国卫生检验杂志, 2020, 30(4):472-474. WANG Xiaohua, CHEN Xiaojun. Research on the miR-25 and miR-141 levels of peripheral blood exosome in patients with liver cancer and the diagnostic efficiency of combined detection [J]. Chinese Journal of Health Laboratory Technology, 2020,30 (4): 472-474.
[16] RASHED M H, BAYRAKTAR E, HELAL G K, et al.Exosomes: from garbage Bins to promising therapeutic targets[J]. International Journal of Molecular Sciences,2017, 18(3): 538-563.
[17] SHTAM T A, KOVALEV R A, VARFOLOMEEVA E Y, et al. Exosomes are natural carriers of exogenous siRNA to human cells in vitro[J]. Cell Communication and Signaling : CCS, 2013, 11: 88.
[18] JAFRI M, AL-QAHTANI M H, SHAY J W. Role of miRNAs in human cancer metastasis: Implications for therapeutic intervention[J]. Seminars in Cancer Biology, 2017, 44: 117-131.
[19] ZUO Duo, CHEN Liwei, LIU Xiaoqian, et al.Combination of miR-125b and miR-27a enhances sensitivity and specificity of AFP-based diagnosis of hepatocellular carcinoma[J]. Tumour Biology: 2016,37(5): 6539-6549.
[20] 陈特, 毕小云, 徐华建, 等. 三种血清标志物联合检测在早期肝细胞肝癌中的诊断价值[J]. 重庆医学,2017, 46(35):4923-4926, 4929. CHEN Te, BI Xiaoyun, XU Huajian, et al. Diagnostic value of GP73,AFP and AFU combined detection in early hepatocelluar carcinoma [J]. Chongqing Medicine, 2017,46 (35): 4923-4926, 4929.

相似文献/References:

[1]翁震,何杨.外泌体蛋白质组学分析在心血管疾病中应用的研究进展[J].现代检验医学杂志,2018,33(04):157.[doi:10.3969/j.issn.1671-7414.2018.04.044]
　WENG Zhen,HE Yang.Research Progress of Proteomic Analysis of Exosomes and Its Application in Cardiovascular Disease[J].Journal of Modern Laboratory Medicine,2018,33(04):157.[doi:10.3969/j.issn.1671-7414.2018.04.044]
[2]蒲双双,李金星.外泌体在疾病实验诊断和临床治疗中的研究进展[J].现代检验医学杂志,2018,33(04):160.[doi:10.3969/j.issn.1671-7414.2018.04.045]
　PU Shuang-shuang,LI Jin-xing.Research Progress of Exosome in the Laboratory Diagnosis and Clinic Treatment of Disease[J].Journal of Modern Laboratory Medicine,2018,33(04):160.[doi:10.3969/j.issn.1671-7414.2018.04.045]
[3]蒲双双.外泌体的提取、鉴定和保存方法研究进展[J].现代检验医学杂志,2018,33(05):157.[doi:10.3969/j.issn.1671-7414.2018.05.044]
　PU Shuang-shuang.Research Progress in Isolation,Identification and Preservation of Exosomes[J].Journal of Modern Laboratory Medicine,2018,33(04):157.[doi:10.3969/j.issn.1671-7414.2018.05.044]
[4]张翠平,陈婷婷,王成,等.儿童原发性肾病综合征患者尿液外泌体miR-23b-3p水平变化及其临床意义[J].现代检验医学杂志,2019,34(01):1.[doi:10.3969/j.issn.1671-7414.2019.01.001]
　ZHANG Cui-ping,CHEN Ting-ting,WANG Cheng,et al.Levels and Its Significance of Urinary Exosomal miR-23b-3p in Children with Nephrotic Syndrome[J].Journal of Modern Laboratory Medicine,2019,34(04):1.[doi:10.3969/j.issn.1671-7414.2019.01.001]
[5]樊春荔,何燕娟.缺血性脑卒中和短暂性脑缺血发作患者血清miR-23b-3p 水平变化的比较研究[J].现代检验医学杂志,2020,35(05):51.[doi:10.3969/j.issn.1671-7414.2020.05.013]
　FAN Chun-li,HE Yan-juan.Altered Levels of Serum miR-23b-3p in Patients with Ischemic Stroke and Transient Ischemic Attack[J].Journal of Modern Laboratory Medicine,2020,35(04):51.[doi:10.3969/j.issn.1671-7414.2020.05.013]
[6]张筱东,谢星星,李　佳,等.血清外泌体 miR-221 和 miR-378 水平检测在胃癌诊断中的应用研究[J].现代检验医学杂志,2020,35(06):52.[doi:doi:10.3969/j.issn.1671-7414.2020.06.013]
　ZHANG Xiao-dong,XIE Xing-xing,LI Jia,et al.Application Study of Serum Exosome miR-221 and miR-378 Detection in theDiagnosis of Gastric Cancer[J].Journal of Modern Laboratory Medicine,2020,35(04):52.[doi:doi:10.3969/j.issn.1671-7414.2020.06.013]
[7]夏艳艳,沈　瀚,许红攀,等.液体活检技术在肿瘤诊断中的最新研究进展[J].现代检验医学杂志,2021,36(03):157.[doi:10.3969/j.issn.1671-7414.2021.03.037]
　XIA Yan-yan,SHEN Han,XU Hong-pan,et al.Latest Research Progress of Liquid Biopsy in Tumor Diagnosis[J].Journal of Modern Laboratory Medicine,2021,36(04):157.[doi:10.3969/j.issn.1671-7414.2021.03.037]
[8]刘先宁,汪　耀,潘士印,等.人角膜基质间充质干细胞外泌体的分离制备及鉴定[J].现代检验医学杂志,2021,36(05):114.[doi:10.3969/j.issn.1671-7414.2021.05.025]
　LIU Xian-ning,WANG Yao,PAN Shi-yin,et al.Isolation and Identification of Exosomes Derived from Human CornealStromal Mesenchymal Stem Cells[J].Journal of Modern Laboratory Medicine,2021,36(04):114.[doi:10.3969/j.issn.1671-7414.2021.05.025]
[9]黄德发,张文娟,陈　杰,等.三种方法提取血清外泌体的比较研究[J].现代检验医学杂志,2021,36(06):139.[doi:10.3969/j.issn.1671-7414.2021.06.030]
　HUANG De-fa,ZHANG Wen-juan,CHEN Jie,et al.Comparative Study on Three Methods for Extraction of Serum Exosomes[J].Journal of Modern Laboratory Medicine,2021,36(04):139.[doi:10.3969/j.issn.1671-7414.2021.06.030]
[10]沈伟兴a,汤佳瑾b,傅　鹏a.2型糖尿病肾病患者外周血 microRNA-155水平与肾小球滤过率的相关性研究[J].现代检验医学杂志,2022,37(01):12.[doi:10.3969/j.issn.1671-7414.2022.01.003]
　SHEN Wei-xinga,TANG Jia-jinb,FU Penga.Correlation between microRNA-155 Level in Peripheral Blood and Glomerular Filtration Rate in Patients with Type 2 DiabeticNephropathy[J].Journal of Modern Laboratory Medicine,2022,37(04):12.[doi:10.3969/j.issn.1671-7414.2022.01.003]
[11]邰国梅,郭　京,王高仁.胸部放疗患者血浆外泌体中miR-7-5p与miR-17-5p的表达及临床意义[J].现代检验医学杂志,2021,36(04):35.[doi:10.3969/j.issn.1671-7414.2021.04.008]
　TAI Guo-mei,GUO Jing,WANG Gao-ren.Expression and Clinical Significance of miR-7-5p and miR-17-5p in PlasmaExosomes of Patients with Thoracic Radiotherapy[J].Journal of Modern Laboratory Medicine,2021,36(04):35.[doi:10.3969/j.issn.1671-7414.2021.04.008]
[12]陆 健,冯 萍,吴 静,等.类风湿关节炎活动期患者血浆外泌体miRNAs 差异表达筛选与生物信息学分析及验证[J].现代检验医学杂志,2024,39(02):62.[doi:10.3969/j.issn.1671-7414.2024.02.012]
　LU Jian,FENG Ping,WU Jing,et al.Bioinformatics Analysis and Validation of Differential Expression of miRNAs in Plasma Exosomes from Patients with Active Rheumatoid Arthritis[J].Journal of Modern Laboratory Medicine,2024,39(04):62.[doi:10.3969/j.issn.1671-7414.2024.02.012]

备注/Memo

备注/Memo:
基金项目：新疆维吾尔自治区卫生健康青年医学科技人才专项科研项目（WYWY-202010）。
作者简介：王路(1989-)，女，硕士研究生，主治医师，主要从事分子诊断方面的研究，E-mail：451918554@qq.com。
通讯作者：伏建峰，男，博士生导师，E-mail：dxpjf@163.com。
更新日期/Last Update: 1900-01-01