[1]刘 鑫,沈 锋,刘 静,等.食管癌患者肠道菌群分布特征与ERCC1基因rs3212986多态性的相关性研究[J].现代检验医学杂志,2026,41(02):105-110.[doi:10.3969/j.issn.1671-7414.2026.02.018]
 LIU Xin,SHEN Feng,LIU Jing,et al.Association between Intestinal Flora Distribution Characteristics with the ERCC1 rs3212986 Polymorphism in Esophageal Cancer Patients[J].Journal of Modern Laboratory Medicine,2026,41(02):105-110.[doi:10.3969/j.issn.1671-7414.2026.02.018]
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食管癌患者肠道菌群分布特征与ERCC1基因rs3212986多态性的相关性研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年02期
页码:
105-110
栏目:
论著
出版日期:
2026-03-15

文章信息/Info

Title:
Association between Intestinal Flora Distribution Characteristics with the ERCC1 rs3212986 Polymorphism in Esophageal Cancer Patients
文章编号:
1671-7414(2026)02-105-06
作者:
刘 鑫a沈 锋b刘 静b周 莹b邓玉兰b余忠林c丁凤娇b
安康市中心医院 a.肿瘤内科;b.检验科;c.胸外科,陕西安康 725000
Author(s):
LIU Xina, SHEN Fengb, LIU Jingb, ZHOU Yingb, DENG Yulanb, YU Zhonglinc, DING Fengjiaob
a.Department of Oncology; b. Department of Clinical Laboratory; c. Department of Thoracic Surgery, Ankang Central Hospital, Shaanxi Ankang 725000, China
关键词:
食管癌肠道菌群切除修复交叉互补基因1基因多态性
分类号:
R735.1;R730.4
DOI:
10.3969/j.issn.1671-7414.2026.02.018
文献标志码:
A
摘要:
目的分析食管癌患者肠道菌群分布特征与切除修复交叉互补基因1(ERCC1)基因rs3212986多态性的相关性研究。方法选取安康市中心医院2021年4月~2024年4月收治的93例食管癌患者为观察组,同期70例健康志愿者为对照组,采用16S核糖体DNA(16SrDNA)鉴定技术检测肠道菌群,分析肠道菌群Beta、Alpha多样性、物种组成及组件丰度的变化,对比ERCC1rs3212986位点分布状况;Spearman相关分析肠道菌群与ERCC1rs3212986多态性的关系。结果与对照组相比,观察组基于丰度的覆盖估计值(ACE)指数、肠球菌属、酵母菌属、大肠埃希菌升高(t=2.475~12.188),覆盖率(Goods_coverage)指数、香农(Shannon)指数、Chao1丰富度(Chaol)指数、辛普森(Simpson)指数、乳杆菌属、双歧杆菌属、消化球菌属和葡萄球菌属降低(t=2.689~27.899),差异具有统计学意义(均P<0.05)。主坐标分析(PCoA)图显示,观察组与对照组样本整体相距较远,群落结构存在差异,相比对照组属水平,观察组栖粪杆菌属、双歧杆菌属比例降低,从门水平分析观察组放线菌门、拟杆菌门比例提升,疣微菌门、厚壁菌门丰度降低(χ2=42.363、25.494,均P<0.05)。且与对照组相比,观察组ERCC1rs3212986位点AA基因型、等位基因A频率升高,差异具有统计学意义(χ2=33.951、27.866,均P<0.05);Spear-man秩相关性分析显示ERCC1rs3212986A等位基因与放线菌、梭杆菌属、变形菌门呈正相关(r=0.173、0.407、0.202,均P<0.05)。与拟杆菌门、厚壁菌门呈负相关(r=-0.337、-0.219,均P<0.05)。结论在食管癌患者中肠道菌群分布特征异常表达,并发现部分肠道菌群分布特征可能与食管癌ERCC1rs3212986多态性有关。
Abstract:
Objective To analyze the correlation between the distribution characteristics of intestinal flora and the rs3212986 polymorphism of the excision repair cross complement 1 (ERCC1) gene in esophageal cancer patients. Methods 93 esoph-ageal cancer patients admitted to Ankang Central Hospital from April 2021 to April 2024 were selected as the observation group, and 70 healthy volunteers were selected as the control group during the same period. 16S ribosomal DNA (16SrDNA) sequencing technology was used to detect the intestinal flora. The changes in intestinal flora Beta, Alpha diversity, species com-position and component abundance changes were analyzed and the distribution status of ERCC1 rs3212986 loci was compared. Spearman correlation coefficient analysis was used to examine the relationship between intestinal flora and ERCC1 rs3212986 polymorphism. Results Compared to the control group, the observation group had higher Abundance-based Coverage Esti-mator (ACE) index, Enterococcus spp , Saccharomyces spp , and Escherichia coli spp (t=2.475~12.188), while the coverage index (Goods_coverage), Shannon index, Chao1 enrichment index (Chaol), Simpson index (Simpson), Lactobacillus spp , Bi-fidobacterium spp, Digestive Coccus spp, Staphylococcus spp decreased (t=2.689~27.899), with statistically significant differ-ences (all P<0.05). The Principal Coordinate Analysis (PCoA) plots showed that the overall distance between samples in the observation group and the control group was relatively large, indicating distinct community structure. Compared to the control group at the genus level, the proportions of the peritrophic E. faecalis and Bifidobacterium spp decreased in the observation group. At the phylum level, the proportions of the Actinobacteria and Bacteroidetes increased, while the abundance of the Wart Micronuclei and Thick-walled Bacteria decreased(χ2=46.363, 25.494, all P<0.05). Furthermore, compared with the control group, the observation group exhibited a statistically significant increase in the frequency of the A allele at the ERCC1 rs3212986 AA genotype (χ2=33.951, 27.866, all P<0.05). Spearman’s rank correlation analysis showed that the ERCC1 rs3212986 A allele posi-tively correlated with the phyla of Actinobacteria, Clostridium, and Proteobacteria (r=0.173, 0.407, 0.202, all P<0.05), while negatively correlated with Bacteroidetes and Firmicutes (r=-0.337,-0.219, all P<0.05). Conclusions Abnormal intestinal flora distribution features ware observed in patients with esophageal cancer, and some of the intestinal flora distribution features may be associated with the ERCC1 rs3212986 polymorphism in esophageal cancer.

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备注/Memo

备注/Memo:
基金项目:陕西省卫生健康科研基金项目(项目编号:2022C007)。
作者简介:刘鑫(1981-),男,本科,主治医生,研究方向:肿瘤综合治疗,E-mail: ya7057924@163.com。
通讯作者:丁凤娇(1989-),女,硕士,副主任医师,研究方向:临床检验诊断学。
更新日期/Last Update: 2026-03-15