[1]杨晓燕,张平安,牛志立,等.慢性丙型肝炎患者外周血单个核细胞中干扰素刺激基因及相关信号通路基因表达的研究[J].现代检验医学杂志,2017,32(03):18-21,25.[doi:10.3969/j.issn.1671-7414.2017.03.005]
 YANG Xiao-yan,ZHANG Ping-an,NIU Zhi-li,et al.Study on Expression Interferon Stimulating Gene and Related Signal Pathway Gene in Peripheral Blood Mononuclear Cells of Patients with Chronic Hepatitis C[J].Journal of Modern Laboratory Medicine,2017,32(03):18-21,25.[doi:10.3969/j.issn.1671-7414.2017.03.005]
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慢性丙型肝炎患者外周血单个核细胞中干扰素刺激基因及相关信号通路基因表达的研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年03期
页码:
18-21,25
栏目:
论著
出版日期:
2017-05-25

文章信息/Info

Title:
Study on Expression Interferon Stimulating Gene and Related Signal Pathway Gene in Peripheral Blood Mononuclear Cells of Patients with Chronic Hepatitis C
文章编号:
1671-7414 (2017)03-018-05
作者:
杨晓燕张平安牛志立王方平
武汉大学人民医院检验科,武汉 430060
Author(s):
YANG Xiao-yanZHANG Ping-anNIU Zhi-liWANG Fang -ping
Department of Clinical Laboratory,Renmin Hospital of Wuhan Univ ersity,Wuhan 430060,China
关键词:
慢性丙型肝炎干扰素刺激基因I型干扰素实时荧光定量聚 合酶链反应
分类号:
R512.63;Q786
DOI:
10.3969/j.issn.1671-7414.2017.03.005
文献标志码:
A
摘要:
目的 观察慢性丙型肝炎(chronic hepatitis C,CHC)患者 外周血单个核细胞(peripheral blood mononuclear cells,PBMC)中干扰素刺激基因(stimu lator of interferon genes,STING)、维甲酸诱导基因I(retinoic acid inducible gene 1,RIG-1),以及I型干扰素α(Interferon-α,IFN-α)和β(IFN-β)mRNA的表达,为 治疗CHC提供新思路。方法 选择2016年1月~2017年1月在武汉大学人 民医院感染科未经治疗的CHC患者113例,以及同期进行体检的健康人94例,通过实时荧光定 量PCR检测STING,RIG-1样受体,以及IFN-α和IFN-β mRNA表达,用2-ΔΔCt 的方法计算相对表达量,并对计算结果进行统计学分析。结果 CH C患者外周血中STING,RIG-1,IFN-α和IFN-β mRNA的表达分别是健康对照组的0.018, 0.361,0.578和0.573倍,两组间的比较差异均有统计学意义(t=8.28,4.26,2.18 和2.07,均P<0.05);健康人STING与IFN-α mRNA,IFN-β mRNA表达呈正相关性(r =0.487,0.207,均P<0.05);CHC患者STING与IFN-α mRNA,IFN-β mRNA表达无相 关性(r=0.174,0.091,均P<0.05);健康人STING与RIG-1 mRNA表达呈低正相关性 (r=0.222,P<0.05),CHC患者STING与RIG-1 mRNA表达无相关性(r=-0.029,P >0.05)。结论 与健康人相比,CHC患者STING,RIG-1,IFN-α和 IFN-β mRNA表达均降低,且在健康人中STING基因与RIG-1及干扰素基因存在正相关性, 而CHC中无相关性。
Abstract:
Objective To detect mRNA expressions of STING a nd type I interferons(IFN-α and IFN-β) in peripheral blood mononuclear cells (PBMCs) of patients with chronic hepatitis C(CHC).Methods 〖WT5”B Z〗113 patients with CHC and 94 healthy controls were collected from Renmin Hosp ital of Wuhan University during January 2015 and January 2016.Expressions of mR NA of STING,RIG-1,IFN-α and IFN-β were detected by real-time quantitativ e PCR,and their relative expression values were obtained by 2-ΔΔCt method and the results were statistically analyzed.Results 〖WT5” BZ〗The expression of mRNA of STING,RIG-1,IFN-α and IFN-β in CHC were 0 .018,0.361,0.578 and 0.573 times than its in healthy controls respectively and the differences were statistically significant (t=8.28,4.26,2.18 a nd 2.07,all P<0.05).Pearson correlation analysis showed that mRNA expres sions of STING in healthy controls were positively correlated with that of IFN- α mRNA and IFN -β mRNA (r=0.487 and 0.207,all P<0.05),which had no correlation in CHC (r=0.174 and 0.091,all P>0.05).The expressi ons of STING mRNA was positively correlated with that of RIG-1 mRNA in healthyc ontrols (r=0.222,P<0.05),but there was no correlation of expression in CHC between STING mRNA and RIG-1 mRNA (r=-0.029,P>0.05).Conc lusion Compared with healthy controls,the expression of STING,RIG -1,IFN-α and IFN-β mRNA were all reduced.There was positive correlation b etween STING and RIG-1,IFN-α and IFN-β in healthy controls,but no correla tion in CHC.

参考文献/References:

[1] Kanda T.Interferon-free treatment for HCV-infected patients w ith decompensated cirrhosis[J].Hepatol Int,2017,11(1):38-44.
[2] Welch NM,Jensen DM.Pegylated interferon based therapy with second-wave direct-acting antivirals in genotype 1 chronic hepatitis C[J].Liver Int,20 15,35(Suppl 1):11-17.
[3] Coppola N,De Pascalis S,Onorato L,et al.Hepatitis B virus and hepatit is C virus infection in healthcare workers[J].World J Hepatol,2016,8(5):27 3-281.
[4] Flisiak R,Pogorzelska J,Flisiak-Jackiewicz M.He-patitis C:efficacy and safety in real life[J].Liver Int,2017,37(Suppl 1):26-32.
[5] 王方平,张平安,牛志立.固有免疫和适应性免疫与慢性丙肝的研究进展[J].现 代检验医学杂志,2015,30(6):6-9. Wang FP,Zhang PA,Niu ZL.Studies about innate and adaptive immune responses on chronic hepatitis C[J].Journal of Modern Laboratory Medicine,20 15,30(6):6-9.
[6] Oshiumi H,Matsumoto M,Seya T.Innate immune res-ponses against viral i nfection and its suppression by viral proteins[J].Yakugaku Zasshi,2013,133( 3):323-328.
[7] Oshiumi H,Matsumoto M,Seya T.Innate immune response to RNA virus infec tion[J].Uirusu,2011,61(2):153-161.
[8] Oshiumi H,Funami K,Aly HH,et al.Multi-step regulation of interferon i nduction by hepatitis C virus[J].Arch Immunol Ther Exp (Warsz),2013,61(2): 127-138.
[9] Liu Y,Goulet ML,Sze A,et al.RIG-I-Mediated STING upregulation restri cts herpes simplex virus 1 infection[J].J Virol,2016,90(20):9406-9419.〖 ZK)〗
[10] Cheng Y,Sun Y,Wang H,et al.Chicken STING mediates activation of the IFN gene independently of the RIG-I gene[J].J Immunol,2015,195(8): 3922-3936.
[11] 中华医学会肝病学分会,中华医学会感染病学分会.丙型肝炎防治指南(2015年更 新版)[J].中华实验和临床感染病杂志(电子版),2015,9(5):590-607. Chinese Society of Hepatology and Chinese Society of Infectious Diseases, Chinese Medical Association.The guideline of prevention and treatment for chron ic hepatitis C(2015 update)[J].Chinese Journal of Experimental and Clinical I nfectious Diseases(Electronic Edition),2015,9(5):590-607.
[12] Pfaffl MW.A new mathematical model for relative quantification in real -time RT-PCR[J/OL].Nucleic Acids Res,2001,29(9):e45.
[13] Livak KJ,Schmittgen TD.Analysis of relative gene expression data usin g real-time quantitative PCR and the 2-Delta Delta C (T)method[J].Methods, 2001,25(4):402-408.
[14] Konno H,Barber GN.The STING controlled cytosolic-DNA activated innate immune pathway and microbial disease[J].Microbes Infect,2014,16(12):998- 1001.
[15] Ran Y,Shu HB,Wang YY.MITA/STING:a central and multifaceted mediator in innate immune response[J].Cytokine Growth Factor Rev,2014,25(6):631-63 9.
[16] Liu S,Cai X,Wu J,et al.Phosphorylation of innate immune adaptor prot eins MAVS,STING and TRIF induces IRF3 activation[J].Science,2015,347(6227) :aaa 2630.
[17] Atianand MK,Fitzgerald KA.Molecular basis of D-NA recognition in the immune system[J].J Immunol,2013,190(5):1911-1918.
[18] Cao X,Ding Q,Lu J,et al.MDA5 plays a critical ro-le in interferon r esponse during hepatitis C virus infection[J].J Hepatol,2015,62(4):771-77 8.
[19] Biacchesi S,Mérour E,Lamoureux A,et al.Both ST-ING and MAVS fish or thologs contribute to the induction of interferon mediated by RIG-I[J].PLoS One,2012,7(10):e47737.
[20] Carroll EC,Jin L,Mori A,et al.The vaccine adjuvant chitosan promotes cellular immunity via DNA sensor cGAS-STING-Dependent induction of type I inte rferons[J].Immunity,2016,44(3):597-608.
[21] Yang D,Liu N,Zuo C,et al.Innate host response in primary human hepato cytes with hepatitis C virus infection[J].PLoS One,2011,6(11):e27552.
[22] Zevini A,Olagnier D,Hiscott J.Crosstalk between cytoplasmic RIG-I an d STING sensing pathways[J].Trends Immunol,2017,38(3):194-205.
[23] Welsch C,Haselow K,Gouttenoire J,et al.Hepatitis C virus variants re sistant to macrocyclic NS3-4A inhibitors subvert IFN-β-induction by efficien t MAVS cleavage[J].J Hepatol,2015,62(4):779-784.
[24] Parera M,Martrus G,Franco S,et al.Canine hepacivirus NS3 serine prot ease can cleave the human adaptor proteins MAVS and TRIF[J/OL].PLoS One,2012 ,7(8):e42481.

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备注/Memo

备注/Memo:

作者简介:杨晓燕(1992-),女,在读研究生,主要从 事免疫遗传学研究,E-mail:707298774@qq.com。
通讯作者:张平安,男,副教授,E-mail: zhangpingan927@16 3.com。
更新日期/Last Update: 2017-06-25