[1]程 亮,王 静,高志华,等.哮喘儿童血清外泌体中miR-7-5p 表达水平与短期预后的相关性研究[J].现代检验医学杂志,2024,39(05):58-62.[doi:10.3969/j.issn.1671-7414.2024.05.011]
　CHENG Liang,WANG Jing,GAO Zhihua,et al.Study on the Correlation between Serum Exosomal miR-7-5p Levels and Short-Term Prognostic Outcomes in Children with Asthma[J].Journal of Modern Laboratory Medicine,2024,39(05):58-62.[doi:10.3969/j.issn.1671-7414.2024.05.011]
点击复制

哮喘儿童血清外泌体中miR-7-5p 表达水平与短期预后的相关性研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年05期
页码:
58-62
栏目:
论著
出版日期:
2024-09-15

文章信息/Info

Title:
Study on the Correlation between Serum Exosomal miR-7-5p Levels and Short-Term Prognostic Outcomes in Children with Asthma
文章编号:
1671-7414（2024）05-058-05
作者:
程 亮1; 王 静2; 高志华1; 李一洁1; 张润春2
（1. 唐山职业技术学院附属医院儿科，河北唐山 063000；2. 唐山市妇幼保健院儿科，河北唐山 063000）
Author(s):
CHENG Liang1;  WANG Jing2;  GAO Zhihua1;  LI Yijie1;  ZHANG Runchun2
（1.Department of Pediatrics, Tangshan Vocational & Technical College Affiliated Hospital, Hebei Tangshan 063000， China；2.Department of Pediatrics，Tangshan Maternal and Child Health Hospital, Hebei Tangshan 063000，China）
关键词:
外泌体; 微小RNA-7-5p; 哮喘; 儿童
分类号:
R562.25；R392.11
DOI:
10.3969/j.issn.1671-7414.2024.05.011
文献标志码:
A
摘要:
目的 分析哮喘儿童血清外泌体中微小RNA（microRNA，miR）-7-5p 表达水平与短期预后的关系。方法 选取2021 年10 月～ 2023 年5 月唐山职业技术学院附属医院的132 例哮喘儿童（哮喘组）和30 例健康儿童（对照组）作为研究对象。根据儿童哮喘控制测试结果将哮喘儿童分为预后不良组（n=41）和预后良好组（n=91）。比较各组的过敏史、用力肺活量（forced vital capacity，FVC）、呼出气一氧化氮（fractional exhaled nitric oxide，FeNO）和miR-7-5p 等水平。用Logistic 回归分析哮喘儿童短期预后的风险因素。用Kappa 检验、受试者工作特征（receiver operatingcharacteristic，ROC）曲线和平均绝对误差（mean absolute error，MAE）值评价各指标判断哮喘儿童短期预后的价值。结果 哮喘组的miR-7-5p[0.53（0.49，0.64）] 水平低于对照组[1.03（0.97，1.10）]，差异具有统计学意义(Z=8.548，P ＜ 0.001)。预后不良组的过敏史占比（68.29%）和FeNO[38（36，41）ppb] 水平高于预后良好组[46.15%，35（30，40）ppb]，FVC[1.86（1.84，2.05）L] 和miR-7-5p[0.47（0.43，0.52）] 水平低于预后良好组[1.94（1.87，2.06）L，0.60（0.50，0.68）]，差异具有统计学意义（χ2/Z=2.854 ～ 6.450，均P ＜ 0.05）。多因素Logistic 回归分析结果显示，过敏史（OR=3.388，95%CI：1.328 ～ 8.643）和高FeNO（OR=1.161，95%CI：1.064 ～ 1.267）水平是哮喘儿童短期预后的独立危险因素(P ＜ 0.01），高miR-7-5p（OR=0.090，95%CI：0.033 ～ 0.248）水平是哮喘儿童短期预后的独立保护因素（P ＜ 0.01）。miR-7-5p+ 过敏史+FeNO 判断哮喘儿童短期预后的ROC 曲线下面积为0.904，大于miR-7-5p（0.851，Z=2.097，P=0.036）和过敏史+FeNO（0.726，Z=4.239，P ＜ 0.001）。miR-7-5p+ 过敏史+FeNO 的Kappa 值为0.67，大于miR-7-5p（0.44）和过敏史+FeNO（0.41）；MAE 值为0.022，小于miR-7-5p（0.067）和过敏史+FeNO（0.035）。结论 哮喘儿童血清外泌体中miR-7-5p 表达水平高与其短期预后良好有关。miR-7-5p，过敏史和FeNO 联合对哮喘儿童短期预后评估有一定价值。
Abstract:
Objective To analyze the relationship between serum exosomal microRNA (miR) -7-5p expression levels in children with asthma and their short-term prognosis. Methods A total of 132 asthmatic children (asthma group) and 30 healthy children (control group) from Tangshan Vocational & Technical College Affiliated Hospital between October 2021 and May 2023 were included as study subjects. These asthmatic children were categorized into a good prognosis group（n=91）and a poor prognosis group （n=41）based on their Childhood Asthma Control Test results. The levels of allergy history, forced vital capacity (FVC), fractional exhaled nitric oxide (FeNO), and miR-7-5p level among groups were compared, and logistic regression analysis was conducted to identify risk factors for the short-term prognosis of asthmatic children. The Kappa test, receiver operating characteristic (ROC) curve, and mean absolute error (MAE) value were used to evaluate indicators in predicting short-term prognosis in children with asthma. Results The level of miR-7-5p in the asthma group[0.53（0.49，0.64）] was lower than that in the control group [1.03(0.97，1.10)], with significant difference (Z=8.548, P<0.001). The poor prognosis group exhibited a higher proportion of allergy history（68.29%） and FeNO[38(36, 41) ppb] levels, and lower FVC [1.86(1.84, 2.05) L] and miR-7-5p levels [0.47(0.43, 0.52) ] compared to the good prognosis group[46.15%，35(30，40) ppb，1.94(1.87, 2.06) L，0.60(0.50, 0.68)], and the differences were significant (χ2/Z=2.854 ～ 6.450, all P<0.05). Multivariate logistic regression analysis revealed that a history of allergies (OR=3.388, 95% CI: 1.328 ～ 8.643, P=0.011) and high FeNO levels (OR=1.161, 95% CI: 1.064 ～ 1.267, P=0.001) were independent risk factors, while a high level of miR-7-5p (OR=0.090, 95% CI: 0.033 ～ 0.248, P<0.001) was an independent protective factor for the short-term prognosis of asthmatic children. The area under the ROC curve for miR-7-5p + allergy history + FeNO in predicting the short-term prognosis of asthmatic children was 0.851, which was higher than that for miR-7-5p alone (0.851, Z=2.097, P=0.036) and allergy history + FeNO (0.726, Z=4.239, P<0.001). The Kappa value for miR-7-5p + allergy history + FeNO was 0.67, which was higher than miR-7-5p (0.44) and allergy history + FeNO (0.41). The MAE for miR-7-5p + allergy history + FeNO was 0.022, which was lower than miR-7-5p alone (0.067) and the combination of allergy history + FeNO (0.035). Conclusion Elevated level of miR-7-5p in the serum exosomes of asthmatic children is linked to a favorable short-term prognosis. The combination of miR-7-5p, allergy history, and FeNO may have certain value in evaluating the short-term prognosis of asthmatic children.

参考文献/References:

[1] SHIPP C L, GERGEN P J, GERN J E, et al. Asthma management in children[J].Journal of Allergy and Clinical Immunology in Practice, 2023, 11(1): 9-18.
[2] 中华儿科杂志编辑委员会, 中华医学会儿科学分会呼吸学组, 中国医师协会儿科医师分会儿童呼吸专业委员会. 儿童支气管哮喘规范化诊治建议（2020年版）[J]. 中华儿科杂志, 2020, 58(9):708-717. The Editorial Board, Chinese Journal of Pediatrics, the Subspecialty Group of Respiratory, the Society of Pediatrics, Chinese Medical Association, the Children’s Respiratory Professional Committee, the Society of Pediatrics of Chinese Medical Doctor Association. Recommendations for diagnosis and management of bronchial asthma in children (2020)[J]. Chinese Journal of Pediatrics, 2020, 58(9): 708-717.
[3] WANG Liyan, LIU Xueru. Long noncoding RNA antisense noncoding RNA in the INK4 locus inhibition alleviates airway remodeling in asthma through the regulation of the microRNA-7-5p/early growth response factor 3 axis[J]. Immunity Inflammation and Disease, 2023, 11(4): e823.
[4] TIWARI A, HOBBS B D, LI Jiang, et al. Blood miRNAs are linked to frequent asthma exacerbations in childhood asthma and adult COPD[J]. Non-coding RNA, 2022, 8(2): 27.
[5] PILETTE C. Role of exosomes in allergic asthma: signaling platforms between the epithelium and type 2 immunity[J]. Journal of Allergy and Clinical Immunology, 2021, 148(6): 1478-1480.
[6] V?ZQUEZ-MERA S, MARTELO-VIDAL L, MIGU?NS-SU?REZ P, et al. Serum exosome inflammamiRs are surrogate biomarkers for asthma phenotype and severity[J]. Allergy, 2023, 78(1): 141-155.
[7] 中华医学会儿科学会呼吸学组, 《中华儿科杂志》编辑委员会. 儿童支气管哮喘诊断与防治指南(2016年版)[J]. 中华儿科杂志, 2016, 54(3): 167-181. The Subspecialty Group of Respiratory, the Society of Pediatrics, Chinese Medical Association, the Editorial Board of Chinese Journal of Pediatrics. Guidelines for the diagnosis and prevention of bronchial asthma in children(2016 Edition)[J]. Chinese Journal of Pediatrics, 2016, 54(3): 167-181.
[8] ZHUANG Shaowei, MA Yan, ZENG Yuxiao, et al. METTL14 promotes doxorubicin-induced cardiomyocyte ferroptosis by regulating the KCNQ1OT1-miR-7- 5p-TFRC axis[J]. Cell Biology and Toxicology, 2023, 39(3): 1015-1035.
[9] CHU F, KAPPEL N, AKEL M, et al. Validity of the childhood asthma control test in diverse populations:a systematic review[J]. Pediatric Pulmonology, 2023, 58(5): 1322-1336.
[10] 余文博, 杨磊. 学龄前期儿童哮喘的诊断与治疗研究进展[J]. 新乡医学院学报, 2023, 40(11): 1091-1096. YU Wenbo, YANG Lei. Research progress on diagnosis and treatment of asthma in pre-school children[J]. Journal of Xinxiang Medical University, 2023, 40(11): 1091-1096.
[11] LI Xue, SONG Peige, ZHU Yongjian, et al. The disease burden of childhood asthma in China: a systematic review and meta-analysis[J]. Journal of Global Health, 2020, 10(1): 010801.
[12] WU Chuansha, ZHANG Yunquan, WEI Jing, et al. Associations of early-life exposure to submicron particulate matter with childhood asthma and wheeze in China[J]. JAMA Network Open, 2022, 5(10): e2236003.
[13] LIU Sile, WANG Weiyuan, NING Yue, et al. Exosomemediated miR-7-5p delivery enhances the anticancer effect of Everolimus via blocking MNK/eIF4E axis in non-small cell lung cancer[J]. Cell Death & Disease, 2022, 13(2): 129.
[14] SHANG Yuanyuan, ZHU Zhe, ZHANG Yuanyuan, et al. MiR-7-5p/KLF4 signaling inhibits stemness and radioresistance in colorectal cancer[J]. Cell Death Discovery, 2023, 9(1): 42.
[15] WANG Chong, TANG Zhao, ZHANG Ze, et al. MiR-7-5p suppresses invasion via downregulation of the autophagy-related gene ATG7 and increases chemoresistance to cisplatin in BCa[J]. Bioengineered, 2022, 13(3): 7328-7339.
[16] 王立芹, 陈红营, 隋英, 等. LncRNA OIP5-AS1 靶向miR-7-5p 对LPS 诱导的人支气管上皮细胞损伤的影响[J]. 中国免疫学杂志, 2023, 39(2): 287-290. WANG Liqin, CHEN Hongying, SUI Ying, et al. The effect of lncRNA OIP5-AS1 targeting miR-7-5p on LPS-induced human bronchial epithelial cell injury[J]. Chinese Journal of Immunology, 2023, 39(2): 287-290.
[17] MILLER R L, GRAYSON M H, STROTHMAN K. Advances in asthma: new understandings of asthma’s natural history, risk factors, underlying mechanisms, and clinical management[J]. Journal of Allergy and Clinical Immunology, 2021, 148(6): 1430-1441.
[18] AKAR-GHIBRIL N, CASALE T, CUSTOVIC A, et al. Allergic endotypes and phenotypes of asthma[J]. The Journal of Allergy and Clinical Immunology in Practice, 2020, 8(2): 429-440.
[19] KOUMPAGIOTI D, BOUTOPOULOU B, MORIKI D, et al. Does adherence to the mediterranean diet have a protective effect against asthma and allergies in children? a systematic review[J]. Nutrients, 2022, 14(8): 1618.
[20] 王述红, 邱容, 罗晓斌, 等. 慢性阻塞性肺疾病患者血气分析指标, FeNO, EOS% 水平变化与CAT 评分的相关性研究[J]. 现代检验医学杂志, 2021, 36(4):156-161. WANG Shuhong, QIU Rong, LUO Xiaobin, et al. Correlation between changes of blood gas analysis indexes, FeNO, Eos% and CAT score in patients with chronic obstructive pulmonary disease[J]. Journal of Modern Laboratory Medicine, 2021, 36(4): 156-161.
[21] BUSSE W W, WENZEL S E, CASALE T B, et al. Baseline FeNO as a prognostic biomarker for subsequent severe asthma exacerbations in patients with uncontrolled, moderate-to-severe asthma receiving placebo in the LIBERTY ASTHMA QUEST study: a post-hoc analysis[J]. Lancet. Respiratory Medicine, 2021, 9(10): 1165-1173.

相似文献/References:

[1]翁震,何杨.外泌体蛋白质组学分析在心血管疾病中应用的研究进展[J].现代检验医学杂志,2018,33(04):157.[doi:10.3969/j.issn.1671-7414.2018.04.044]
　WENG Zhen,HE Yang.Research Progress of Proteomic Analysis of Exosomes and Its Application in Cardiovascular Disease[J].Journal of Modern Laboratory Medicine,2018,33(05):157.[doi:10.3969/j.issn.1671-7414.2018.04.044]
[2]蒲双双,李金星.外泌体在疾病实验诊断和临床治疗中的研究进展[J].现代检验医学杂志,2018,33(04):160.[doi:10.3969/j.issn.1671-7414.2018.04.045]
　PU Shuang-shuang,LI Jin-xing.Research Progress of Exosome in the Laboratory Diagnosis and Clinic Treatment of Disease[J].Journal of Modern Laboratory Medicine,2018,33(05):160.[doi:10.3969/j.issn.1671-7414.2018.04.045]
[3]蒲双双.外泌体的提取、鉴定和保存方法研究进展[J].现代检验医学杂志,2018,33(05):157.[doi:10.3969/j.issn.1671-7414.2018.05.044]
　PU Shuang-shuang.Research Progress in Isolation,Identification and Preservation of Exosomes[J].Journal of Modern Laboratory Medicine,2018,33(05):157.[doi:10.3969/j.issn.1671-7414.2018.05.044]
[4]张翠平,陈婷婷,王成,等.儿童原发性肾病综合征患者尿液外泌体miR-23b-3p水平变化及其临床意义[J].现代检验医学杂志,2019,34(01):1.[doi:10.3969/j.issn.1671-7414.2019.01.001]
　ZHANG Cui-ping,CHEN Ting-ting,WANG Cheng,et al.Levels and Its Significance of Urinary Exosomal miR-23b-3p in Children with Nephrotic Syndrome[J].Journal of Modern Laboratory Medicine,2019,34(05):1.[doi:10.3969/j.issn.1671-7414.2019.01.001]
[5]张筱东,谢星星,李　佳,等.血清外泌体 miR-221 和 miR-378 水平检测在胃癌诊断中的应用研究[J].现代检验医学杂志,2020,35(06):52.[doi:doi:10.3969/j.issn.1671-7414.2020.06.013]
　ZHANG Xiao-dong,XIE Xing-xing,LI Jia,et al.Application Study of Serum Exosome miR-221 and miR-378 Detection in theDiagnosis of Gastric Cancer[J].Journal of Modern Laboratory Medicine,2020,35(05):52.[doi:doi:10.3969/j.issn.1671-7414.2020.06.013]
[6]夏艳艳,沈　瀚,许红攀,等.液体活检技术在肿瘤诊断中的最新研究进展[J].现代检验医学杂志,2021,36(03):157.[doi:10.3969/j.issn.1671-7414.2021.03.037]
　XIA Yan-yan,SHEN Han,XU Hong-pan,et al.Latest Research Progress of Liquid Biopsy in Tumor Diagnosis[J].Journal of Modern Laboratory Medicine,2021,36(05):157.[doi:10.3969/j.issn.1671-7414.2021.03.037]
[7]邰国梅,郭　京,王高仁.胸部放疗患者血浆外泌体中miR-7-5p与miR-17-5p的表达及临床意义[J].现代检验医学杂志,2021,36(04):35.[doi:10.3969/j.issn.1671-7414.2021.04.008]
　TAI Guo-mei,GUO Jing,WANG Gao-ren.Expression and Clinical Significance of miR-7-5p and miR-17-5p in PlasmaExosomes of Patients with Thoracic Radiotherapy[J].Journal of Modern Laboratory Medicine,2021,36(05):35.[doi:10.3969/j.issn.1671-7414.2021.04.008]
[8]刘先宁,汪　耀,潘士印,等.人角膜基质间充质干细胞外泌体的分离制备及鉴定[J].现代检验医学杂志,2021,36(05):114.[doi:10.3969/j.issn.1671-7414.2021.05.025]
　LIU Xian-ning,WANG Yao,PAN Shi-yin,et al.Isolation and Identification of Exosomes Derived from Human CornealStromal Mesenchymal Stem Cells[J].Journal of Modern Laboratory Medicine,2021,36(05):114.[doi:10.3969/j.issn.1671-7414.2021.05.025]
[9]黄德发,张文娟,陈　杰,等.三种方法提取血清外泌体的比较研究[J].现代检验医学杂志,2021,36(06):139.[doi:10.3969/j.issn.1671-7414.2021.06.030]
　HUANG De-fa,ZHANG Wen-juan,CHEN Jie,et al.Comparative Study on Three Methods for Extraction of Serum Exosomes[J].Journal of Modern Laboratory Medicine,2021,36(05):139.[doi:10.3969/j.issn.1671-7414.2021.06.030]
[10]王 路,叶 莎,韩 霞,等.循环外泌体miRNA 检测对肝细胞癌临床诊断效能的Meta 分析[J].现代检验医学杂志,2022,37(04):59.[doi:10.3969/j.issn.1671-7414.2021.04.012]
　WANG Lu,YE Sha,HAN Xia,et al.Meta-Analysis of the Diagnostic Value of Circulating Exosomes miRNA Detection for Hepatocellular Carcinoma[J].Journal of Modern Laboratory Medicine,2022,37(05):59.[doi:10.3969/j.issn.1671-7414.2021.04.012]

备注/Memo

备注/Memo:
基金项目：河北省医学科学研究课题计划项目（20221758）。
作者简介：程亮（1982-），女，本科，主治医师，研究方向：儿科疾病诊疗，E-mail: chengliang41223@163.com。
通讯作者：王静（1985-），女，本科，主治医师，研究方向：儿科疾病临床诊治，E-mail: wangjing_8505@163.com。
更新日期/Last Update: 2024-09-15