[1]陈泽衍,马　雯,张立俊,等.荧光免疫层析法检测全血2019-nCoV IgM 和IgG 抗体在新型冠状病毒肺炎诊断中的应用[J].现代检验医学杂志,2020,35(03):84-86.[doi:10.3969/j.issn.1671-7414.2020.03.021]
　CHEN Ze-yan,MA Wen,ZHANG Li-jun,et al.Application of 2019-nCoV IgM and IgG Tests by Fluorescence Immunochromatography in the Diagnosis of 2019-nCoV Infections[J].Journal of Modern Laboratory Medicine,2020,35(03):84-86.[doi:10.3969/j.issn.1671-7414.2020.03.021]
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荧光免疫层析法检测全血2019-nCoV IgM 和IgG 抗体在新型冠状病毒肺炎诊断中的应用 ()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年03期
页码:
84-86
栏目:
新型冠状病毒肺炎检验专题
出版日期:
2020-07-20

文章信息/Info

Title:
Application of 2019-nCoV IgM and IgG Tests by Fluorescence Immunochromatography in the Diagnosis of 2019-nCoV Infections
文章编号:
1671-7414（2020）03-084-04
作者:
陈泽衍1; 马　雯1; 张立俊1; 许玉成2; 申红卫1; 刘　娇1; 梁　琪3; 陈晓静3; 贾兴旺1
(1. 南方医科大学深圳医院检验科，广东深圳 518110；2. 深圳市福田区疾病预防控制中心，广东深圳 518040； 3. 深圳大学总医院检验科，广东深圳 518055）
Author(s):
CHEN Ze-yan1; MA Wen1; ZHANG Li-jun1; XU Yu-cheng2; SHEN Hong-wei1;  LIU Jiao1;  LIANG Qi3;  CHEN Xiao-jing3;  JIA Xing-wang1
(1.Department of Clinical Laboratory, Shenzhen Hospital of Southern Medical University, Guangdong Shenzhen 518110, China ；2.Futian District Center for Disease Control and Prevention, Guangdong Shenzhen 518040, China ；3.Department of Clinical Laboratory, the General Hospital of Shenzhen University,Guangdong Shenzhen 518055, China）
关键词:
荧光免疫层析法; 新型冠状病毒; 2019-nCoV; COVID-19; 免疫球蛋白M; 免疫球蛋白G
分类号:
R373.1；R392.11
DOI:
10.3969/j.issn.1671-7414.2020.03.021
摘要:
目的　探究荧光免疫层析法检测IgM 和IgG 抗体在诊断新型冠状病毒肺炎（COVID-19）患者中的应用价值。 方法　选取2020 年1~2 月在隔离病区收治观察的28 例留观患者作为观察组，均是首次新型冠状病毒（2019-nCoV）咽 拭子核酸检测阳性而第二次以后连续两次核酸检测为阴性的患者，将同期发热门诊排除COVID-19 的30 例发热患者作 为对照组，采用荧光免疫层析法检测所有研究对象的2019-nCoV 免疫球蛋白M（IgM）和免疫球蛋白G（IgG）抗体水平， 采用秩和检验对组间差异进行统计学分析；比较两组患者2019-nCoV IgM 和IgG 的阳性检出率；计算荧光免疫层析法 检测2019-nCoV IgM 和IgG 水平诊断新型冠状病毒肺炎的灵敏度和特异度。结果　观察组2019-nCoV IgM 和IgG 水平 差异有统计学意义（均Z=-6.412，P<0.05）；观察组2019-nCoV IgM 和IgG 的阳性检出率分别为71.4% 和75.0%，联 合检测阳性检出率为96.4%。结论　荧光免疫层析法检测IgM 和IgG 抗体在新型冠状病毒肺炎诊断中具有较高的灵敏 度和特异度，可作为新型冠状病毒感染的有效筛查指标。
Abstract:
Objective　To investigate the value of 2019-nCoV IgM and IgG tests by fluorescence immunochromatography in the diagnosis of 2019-nCoV infections. Methods　From January 2020 to February 2020, 28 patients who were admitted and observed in the isolated area were selected as the observation group.They were the first novel coronavirus (2019-nCoV) nucleic acid test positive for throat swabs, and two times negative for second times after nucleic acid test. Other 30 patients who visited the fever clinic without 2019-nCoV infections during the same period were enrolled as controls. The 2019-nCoV IgM and IgG levels were detected using fluorescence immunochromatography for all the patients. The difference of antibody levels between the two groups was analyzed using Wilcoxon rank sum test. The sensitivity and specificity of IgM and IgG tests in diagnosis of 2019-nCoV infections were also analyzed. Results　The IgM and IgG levels in the 2019-nCoV group was significantly higher than those in controls. The positive detection rate of 2019-nCoV IgM, IgG, and IgM+IgG in the observationcases was 71.4%, 75.0% and 96.4%, respectively. Conclusion　Fluorescence immunochromatography for 2019-nCoV IgM and IgG has achieved high sensitivity and specificity in the diagnosis of 2019-nCoV infections.The combination of IgM and IgG improved the sensitivity and could be used as effective screening and diagnostic indicators.

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备注/Memo

备注/Memo:
收稿日期：2020-03-25 修回日期：2020-04-26作者简介：陈泽衍 (1986- )，男，本科，主管检验师，研究方向：生化及免疫，E-mail：chenzeyan8@163.com。 通讯作者：贾兴旺，副主任技师，E-mail：jiaxingw301@163.com。
更新日期/Last Update: 2020-07-20